IGEM:MIT/2005/Receiver Head Unit: scFv: Difference between revisions

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>Jenny
>Jenny
Line 11: Line 11:
*pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped
*pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped


  FWD: GAATTC GCGGCCGC T TCTAGA GACGTCGTTATGACTCAAAC
  FWD: 5' CATCAT GAATTC TCTAGA GACGTCGTTATGACTCAAAC 3'
      EcoRI   NotI    XbaI      Primer
                EcoRI   XbaI      Primer


  REV: CTGCAG GCGGCCGC T ACTAGT TTATTA CTTATCATCATCATCCTTATAATC GGAGACGGTGACTGAGGTTCC
  REV: 5' GCTCAT CTGCAG ACTAGT TTATTA CTTATCATCATCATCCTTATAATC GGAGACGGTGACTGAGGTTCC 3'
      PstI   NotI      SpeI  Stop          FLAG Tag                Primer
                PstI   SpeI  Stop          FLAG Tag                Primer


==Open Issues/Questions==
==Open Issues/Questions==

Revision as of 09:38, 13 July 2005

POC

Jenny

Function

  • To provide a landing site for ligand.
  • To cause dimerization/conformational change in the Receiver.
  • To transmit signal to Receiver.

Device Depiction

Device Parts

Current Status

  • Primers have been designed for biobrick format.
  • pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped
FWD: 5' CATCAT GAATTC TCTAGA GACGTCGTTATGACTCAAAC 3'
               EcoRI   XbaI       Primer

REV: 5' GCTCAT CTGCAG ACTAGT TTATTA CTTATCATCATCATCCTTATAATC GGAGACGGTGACTGAGGTTCC 3'
                PstI   SpeI   Stop          FLAG Tag                 Primer

Open Issues/Questions

  • TAG (myc) to know that the fluorescein has bound.
  • How to design primers so that the sequence is in frame?

Need Help With

Check to see if primers are correct. Will talk to Kate 7/13.

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