IGEM:MIT/2005/Receiver Head Unit: scFv: Difference between revisions
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*To transmit signal to Receiver. | *To transmit signal to Receiver. | ||
==Device Depiction== | ==Device Depiction== | ||
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[[Image:ScFv.jpg|thumb|100px|scFv]] | [[Image:ScFv.jpg|thumb|100px|scFv]] | ||
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==Device Parts== | ==Device Parts== | ||
Revision as of 10:19, 13 July 2005
POC
Jenny
Function
- To provide a landing site for ligand.
- To cause dimerization/conformational change in the Receiver.
- To transmit signal to Receiver.
Device Depiction
Device Parts
Current Status
- Primers have been designed for biobrick format.
- pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped
FWD: 5' CATCAT GAATTC TCTAGA GACGTCGTTATGACTCAAAC 3'
EcoRI XbaI Primer
REV: 5' GCTCAT CTGCAG ACTAGT TTATTA CTTATCATCATCATCCTTATAATC GGAGACGGTGACTGAGGTTCC 3'
PstI SpeI Stop FLAG Tag Primer
Open Issues/Questions
TAG (myc) to know that the fluorescein has bound.How to design primers so that the sequence is in frame?
Need Help With
Check to see if primers are correct. Will talk to Kate 7/13.
