IGEM:MIT/2005/Receiver Head Unit: scFv: Difference between revisions
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==Device Parts== | ==Device Parts== | ||
<b>scFv</b> | |||
FWD: 5' CATCAT GAATTC GCGGCCGC T TCTAGA GACGTCGTTATGACTCAAAC 3' | |||
rand EcoRI NotI XbaI Primer | |||
REV: 5' GCTCAT CTGCAG GCGGCCGC T ACTAGT TTATTA CTTATCATCATCATCCTTATAATC GGAGACGGTGACTGAGGTTCC 3' | |||
rand PstI NotI SpeI Stop FLAG Tag Primer | |||
<b>BBa_J07012</b> scFv anti-fluorescein [4-4-20][1/3] | |||
Length: 762 Base Pairs Including Part and Barcode | |||
gacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgattttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcagcagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtgacgtcaaactggatgagactggaggaggcttggtgcaacctgggaggcccatgaaactctcctgtgttgcctctggattcacttttagtgactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaaattagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacatgggtatctattactgtacgggttcttactatggtatggactactggggtcaaggaacctcagtcaccgtctcc | |||
==Current Status== | ==Current Status== | ||
*Primers have been designed for biobrick format. | *Primers have been designed for biobrick format. | ||
Revision as of 11:11, 13 July 2005
POC
Jenny
Function
- To provide a landing site for ligand.
- To cause dimerization/conformational change in the Receiver.
- To transmit signal to Receiver.
Device Depiction
-
scFv
Device Parts
scFv
FWD: 5' CATCAT GAATTC GCGGCCGC T TCTAGA GACGTCGTTATGACTCAAAC 3'
rand EcoRI NotI XbaI Primer
REV: 5' GCTCAT CTGCAG GCGGCCGC T ACTAGT TTATTA CTTATCATCATCATCCTTATAATC GGAGACGGTGACTGAGGTTCC 3'
rand PstI NotI SpeI Stop FLAG Tag Primer
BBa_J07012 scFv anti-fluorescein [4-4-20][1/3] Length: 762 Base Pairs Including Part and Barcode
gacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgattttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcagcagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtgacgtcaaactggatgagactggaggaggcttggtgcaacctgggaggcccatgaaactctcctgtgttgcctctggattcacttttagtgactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaaattagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacatgggtatctattactgtacgggttcttactatggtatggactactggggtcaaggaacctcagtcaccgtctcc
Current Status
- Primers have been designed for biobrick format.
- pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped
FWD: 5' CATCAT GAATTC GCGGCCGC T TCTAGA GACGTCGTTATGACTCAAAC 3'
rand EcoRI NotI XbaI Primer
REV: 5' GCTCAT CTGCAG GCGGCCGC T ACTAGT TTATTA CTTATCATCATCATCCTTATAATC GGAGACGGTGACTGAGGTTCC 3'
rand PstI NotI SpeI Stop FLAG Tag Primer
Open Issues/Questions
- How are we moving forward from this?
TAG (myc) to know that the fluorescein has bound.How to design primers so that the sequence is in frame?
Need Help With
Check to see if primers are correct. Will talk to Kate 7/13.
