IGEM:MIT/2005/Receiver Head Unit: scFv

POC

Jenny

Function

• To provide a landing site for ligand.
• To cause dimerization/conformational change in the Receiver.
• To transmit signal to Receiver.

Current Status

• Primers have been designed for biobrick format.
• pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped

FWD: 5' CATCAT GAATTC TCTAGA GACGTCGTTATGACTCAAAC 3'
                EcoRI   XbaI       Primer

REV: 5' GCTCAT CTGCAG ACTAGT TTATTA CTTATCATCATCATCCTTATAATC GGAGACGGTGACTGAGGTTCC 3'
                PstI   SpeI   Stop          FLAG Tag                 Primer

Open Issues/Questions

• TAG (myc) to know that the fluorescein has bound.
• How to design primers so that the sequence is in frame?

Need Help With

Check to see if primers are correct. Will talk to Kate 7/13.