IGEM:MIT/2005/Techniques: Difference between revisions
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==Lab Protocols== | ==Lab Protocols== | ||
*[[../Resuspend Primers To Working Concentration/]] | |||
*[[../Pipetman use, working w/ bacteria, PCR/]] | *[[../Pipetman use, working w/ bacteria, PCR/]] | ||
*[[../Miniprepping DNA, Agarose gels, RE digests/]] | *[[../Miniprepping DNA, Agarose gels, RE digests/]] | ||
Revision as of 20:18, 19 July 2005
Lab Protocols
- [[../Resuspend Primers To Working Concentration/]]
- [[../Pipetman use, working w/ bacteria, PCR/]]
- [[../Miniprepping DNA, Agarose gels, RE digests/]]
- [[../Excising DNA from gels, purifying DNA from gels/]]
- [[../Ligations, Transformations/]]
Others
- Codon Usage
- Translate Tool
- Orf (open reading frame) Finder
- Amino Acid Codes
- [[../Ray's Tools/]]
- Restriction Sites:
- EcoR1
- G AATTC
- CTTAA G
- HaeIII
- GG CC
- CC GG
- BamH1
- G GATCC
- CCTAG G
- Pst1
- CTGCA G
- G ACGTC
- EcoKI
- 5'AAC(N)6GTGC3'
- EcoBI
- 5'TCA(N)8TGCT3'
- Not1
- 5' GCGGCCGC 3'
- EcoR1
- Base pair finder - read the readme first... if you have any questions, ask me..
- Epitope Tagging
- QuantumDotfor detection/illuminescence
