IGEM:MIT/2006/Communications

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Figure 2)
Line 1: Line 1:
===Figure 1===
===Figure 1===
-
*(a) normal e. coli (depict chemical inputs and outputs)
+
Complete system diagram.
-
*(b) final eau d’e coli system (depict chemical inputs and outputs)
+
 +
====Archive====
[[Media:MITiGEM2006Figure1.pdf|Figure 1]] (pdf)
[[Media:MITiGEM2006Figure1.pdf|Figure 1]] (pdf)
===Figure 2===
===Figure 2===
Smell generating devices from precursor.   
Smell generating devices from precursor.   
-
*(a) device diagrams of wintergreen and banana generating devices.
+
*(a) device diagrams of wintergreen and banana generating devices.
 +
**[[Media:MITiGEM2006Figure2a.pdf|Figure 2a]]
*(b) smell test results from Jamboree  
*(b) smell test results from Jamboree  
 +
**[[Media:IGEM06Smelltest.xls|Jamboree smell test data]] (xls)
 +
**[[Media:MITiGEM2006Figure4.pdf|Bar graph of smell test results]] (pdf)
*(c) smell test photo?
*(c) smell test photo?
-
 
-
[[Media:MITiGEM2006Figure2a.pdf|Figure 2a]]
 
====Archive====
====Archive====
Line 17: Line 18:
===Figure 3===
===Figure 3===
-
GC data (banana total synthesis, wintergreen total synthesis, banana with precursor, wintergreen with precursor)
+
Autonomous wintergreen production
 +
*(a) GC data of wintergreen total synthesis  
 +
*(b) GC data of J45120 without precursor
===Figure 4===
===Figure 4===
-
Smell test data (show photo of smell test in action and also bar graphs of results)
+
Growth phase control
 +
*(a) parts and device level depiction
 +
*(b) growth-phase control of FP output
-
[[Media:IGEM06Smelltest.xls|Jamboree smell test data]] (xls)
+
====Archive====
-
 
+
*[[Media:MITiGEM2006Figure5.pdf|Figure 5]] (pdf)
-
[[Media:MITiGEM2006Figure4.pdf|Bar graph of smell test results]] (pdf)
+
===Figure 5===
===Figure 5===
-
Growth phase control (parts and device level depiction)
+
*(a) device diagram of regulated banana device  
-
 
+
*(b) timecourse of banana smell
-
[[Media:MITiGEM2006Figure5.pdf|Figure 5]] (pdf)
+
-
 
+
-
*verify the part numbers of the devices for which there is GC data ...
+
-
 
+
-
J45993 vs J45992 and J45199 vs J45219
+
-
 
+
-
===Figure 6===
+
-
Show data with growth-phase control of FP output
+
-
 
+
-
*ask Barry to make pretty figure for this data
+
-
 
+
-
===Figure 7===
+
-
Show GC data from banana time course
+
===Figure S1===
===Figure S1===
Line 47: Line 38:
===Figure S2===
===Figure S2===
-
Show GC data here (could have chromatograms)GC data for IK cells versus normal E. coli; mint scented bacteria versus IK cells.
+
*(a) GC data for IK cells versus normal E. coli;  
 +
*(b) mint scented bacteria with precursor versus TOP10 with precursor.
 +
*(c) banana scented bacteria with precursor versus TOP10 with precursor.  
==References==
==References==

Revision as of 18:16, 25 January 2008

Contents

Figure 1

Complete system diagram.

Archive

Figure 1 (pdf)

Figure 2

Smell generating devices from precursor.

Archive

Figure 2 (pdf)

Figure 3

Autonomous wintergreen production

  • (a) GC data of wintergreen total synthesis
  • (b) GC data of J45120 without precursor

Figure 4

Growth phase control

  • (a) parts and device level depiction
  • (b) growth-phase control of FP output

Archive

Figure 5

  • (a) device diagram of regulated banana device
  • (b) timecourse of banana smell

Figure S1

Restrospective project timeline. How long did each step take? [from this we can compute how we could do such work faster. We’ll want to work the pace of work into both the introduction and discussion].

Figure S2

  • (a) GC data for IK cells versus normal E. coli;
  • (b) mint scented bacteria with precursor versus TOP10 with precursor.
  • (c) banana scented bacteria with precursor versus TOP10 with precursor.

References

Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."

Use of ATF1 in E. coli

  1. Singh R, Vadlani PV, Harrison ML, Bennett GN, and San KY. . pmid:17891501. PubMed HubMed [Singh-BioprocessBiosysEng-2007]

Methyl salicylate synthesis

  1. Negre F, Kolosova N, Knoll J, Kish CM, and Dudareva N. . pmid:12361714. PubMed HubMed [Negre-ArchBiochemBiophys-2002]
  2. Ross JR, Nam KH, D'Auria JC, and Pichersky E. . pmid:10375393. PubMed HubMed [Ross-ArchBiochemBiophys-1999]
  3. Pott MB, Hippauf F, Saschenbrecker S, Chen F, Ross J, Kiefer I, Slusarenko A, Noel JP, Pichersky E, Effmert U, and Piechulla B. . pmid:15310828. PubMed HubMed [Pott-PlantPhysiol-2004]
  4. Zubieta C, Ross JR, Koscheski P, Yang Y, Pichersky E, and Noel JP. . pmid:12897246. PubMed HubMed [Zubieta-PlantCell-2003]
All Medline abstracts: PubMed HubMed
Personal tools