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| Line 1: |
Line 1: |
| ===Figure 1===
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| Complete system diagram.
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| [[Image:MITiGEM2006Figure1.pdf]] | | Please ask [[User:Reshma P. Shetty|Reshma]] for the latest copy of the paper and figures. |
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| ===Figure 2===
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| Smell generating devices from precursor.
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| [[Image:MITiGEM2006Figure2.pdf]]
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| *(a) device diagrams of wintergreen and banana generating devices.
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| **[[Image:MITiGEM2006Figure2a.pdf|Figure 2a]]
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| *(b) smell test results from Jamboree
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| **[[Image:IGEM06Smelltest.xls|Jamboree smell test data]] (xls)
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| *(c) smell test photo?
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| ===Figure 3===
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| Autonomous wintergreen production
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| *(a) Device and parts level diagram of autonomous wintergreen synthesis
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| *(b) GC data of wintergreen total synthesis
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| *(c) GC data of J45120 without precursor
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| *(d) Pure methyl salicylate standard
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| [[Image:MITiGEM2006Figure3.pdf]]
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| ===Figure 4===
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| Growth phase control
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| *(a) parts and device level depiction
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| *(b) growth-phase control of FP output
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| [[Image:MITiGEM2006Figure4.pdf]]
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| ===Figure 5===
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| *(a) device diagram of regulated banana device
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| *(b) timecourse of banana smell
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| [[Image:MITiGEM2006Figure5.pdf]]
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| [[Image:MITiGEM2006Figure5b.pdf]]
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| ===Figure S1===
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| Restrospective project timeline. How long did each step take? [from this we can compute how we could do such work faster. We’ll want to work the pace of work into both the introduction and discussion].
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| ===Figure S2===
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| *(a) GC data for IK cells versus normal E. coli;
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| *(b) mint scented bacteria with precursor versus TOP10 with precursor.
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| *(c) banana scented bacteria with precursor versus TOP10 with precursor.
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| ==References==
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| Also see the relevant part pages for references.
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| ===osmY Promoter Mehods Section (In order)===
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| book reference:
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| Chasel, M. Gentry D.R., Hernandez, V.J., and Vinella, D. (1996) in Neidhardt, F.C. (ed.) ''Escherichia coli and Salmonella-Cellular and Molecular Biology'', 2nd edition. pp. 1476-1477, ASM Press, Washington, DC.
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| | |
| <biblio>
| |
| #Neidhardt isbn=1555810845
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| #Colland-EMBO-2000 pmid=10856246
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| #Schelhorn pmid=9829938
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| #Yim pmid=8282684
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| </biblio>
| |
|
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|
| ===Odor thresholds=== | | ===Odor thresholds=== |
| Line 67: |
Line 7: |
| *[http://people.ok.ubc.ca/neggers/Chem422A/The%20Odor%20and%20Aroma%20of%20Wine.pdf Isoamyl acetate] | | *[http://people.ok.ubc.ca/neggers/Chem422A/The%20Odor%20and%20Aroma%20of%20Wine.pdf Isoamyl acetate] |
| *[http://www.sciencelab.com/xMSDS-Methyl_salicylate-9927362 Methyl salicylate] | | *[http://www.sciencelab.com/xMSDS-Methyl_salicylate-9927362 Methyl salicylate] |
|
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| ===Use of ATF1 in ''E. coli''===
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| <biblio>
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| #Singh-BioprocessBiosysEng-2007 pmid=17891501
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| </biblio>
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|
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| ===Methyl salicylate synthesis===
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| <biblio>
| |
| #Negre-ArchBiochemBiophys-2002 pmid=12361714
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| #Ross-ArchBiochemBiophys-1999 pmid=10375393
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| #Pott-PlantPhysiol-2004 pmid=15310828
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| #Zubieta-PlantCell-2003 pmid=12897246
| |
| </biblio>
| |
