IGEM:MIT/2006/Communications: Difference between revisions

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===Figure 1===
===Figure 1===
*(a) normal e. coli (depict chemical inputs and outputs)
Complete system diagram.
*(b) final eau d’e coli system (depict chemical inputs and outputs)


====Archive====
[[Media:MITiGEM2006Figure1.pdf|Figure 1]] (pdf)
[[Media:MITiGEM2006Figure1.pdf|Figure 1]] (pdf)


===Figure 2===
===Figure 2===
Smell generating devices from precursor.   
Smell generating devices from precursor.   
*(a) device diagrams of wintergreen and banana generating devices.
*(a) device diagrams of wintergreen and banana generating devices.
**[[Media:MITiGEM2006Figure2a.pdf|Figure 2a]]
*(b) smell test results from Jamboree  
*(b) smell test results from Jamboree  
**[[Media:IGEM06Smelltest.xls|Jamboree smell test data]] (xls)
**[[Media:MITiGEM2006Figure4.pdf|Bar graph of smell test results]] (pdf)
*(c) smell test photo?
*(c) smell test photo?
[[Media:MITiGEM2006Figure2a.pdf|Figure 2a]]


====Archive====
====Archive====
Line 17: Line 18:


===Figure 3===
===Figure 3===
GC data (banana total synthesis, wintergreen total synthesis, banana with precursor, wintergreen with precursor)
Autonomous wintergreen production
*(a) GC data of wintergreen total synthesis  
*(b) GC data of J45120 without precursor


===Figure 4===
===Figure 4===
Smell test data (show photo of smell test in action and also bar graphs of results)
Growth phase control
*(a) parts and device level depiction
*(b) growth-phase control of FP output


[[Media:IGEM06Smelltest.xls|Jamboree smell test data]] (xls)
====Archive====
 
*[[Media:MITiGEM2006Figure5.pdf|Figure 5]] (pdf)
[[Media:MITiGEM2006Figure4.pdf|Bar graph of smell test results]] (pdf)


===Figure 5===
===Figure 5===
Growth phase control (parts and device level depiction)
*(a) device diagram of regulated banana device  
 
*(b) timecourse of banana smell
[[Media:MITiGEM2006Figure5.pdf|Figure 5]] (pdf)
 
*verify the part numbers of the devices for which there is GC data ...
 
J45993 vs J45992 and J45199 vs J45219
 
===Figure 6===
Show data with growth-phase control of FP output
 
*ask Barry to make pretty figure for this data
 
===Figure 7===
Show GC data from banana time course


===Figure S1===
===Figure S1===
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===Figure S2===
===Figure S2===
Show GC data here (could have chromatograms)?  GC data for IK cells versus normal E. coli; mint scented bacteria versus IK cells.
*(a) GC data for IK cells versus normal E. coli;  
*(b) mint scented bacteria with precursor versus TOP10 with precursor.
*(c) banana scented bacteria with precursor versus TOP10 with precursor.  


==References==
==References==

Revision as of 15:16, 25 January 2008

Figure 1

Complete system diagram.

Archive

Figure 1 (pdf)

Figure 2

Smell generating devices from precursor.

Archive

Figure 2 (pdf)

Figure 3

Autonomous wintergreen production

  • (a) GC data of wintergreen total synthesis
  • (b) GC data of J45120 without precursor

Figure 4

Growth phase control

  • (a) parts and device level depiction
  • (b) growth-phase control of FP output

Archive

Figure 5

  • (a) device diagram of regulated banana device
  • (b) timecourse of banana smell

Figure S1

Restrospective project timeline. How long did each step take? [from this we can compute how we could do such work faster. We’ll want to work the pace of work into both the introduction and discussion].

Figure S2

  • (a) GC data for IK cells versus normal E. coli;
  • (b) mint scented bacteria with precursor versus TOP10 with precursor.
  • (c) banana scented bacteria with precursor versus TOP10 with precursor.

References

Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."

Use of ATF1 in E. coli

  1. Singh R, Vadlani PV, Harrison ML, Bennett GN, and San KY. Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferases AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients. Bioprocess Biosyst Eng. 2008 Jun;31(4):299-306. DOI:10.1007/s00449-007-0159-3 | PubMed ID:17891501 | HubMed [Singh-BioprocessBiosysEng-2007]

Methyl salicylate synthesis

  1. Negre F, Kolosova N, Knoll J, Kish CM, and Dudareva N. Novel S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme responsible for biosynthesis of methyl salicylate and methyl benzoate, is not involved in floral scent production in snapdragon flowers. Arch Biochem Biophys. 2002 Oct 15;406(2):261-70. DOI:10.1016/s0003-9861(02)00458-7 | PubMed ID:12361714 | HubMed [Negre-ArchBiochemBiophys-2002]
  2. Ross JR, Nam KH, D'Auria JC, and Pichersky E. S-Adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme involved in floral scent production and plant defense, represents a new class of plant methyltransferases. Arch Biochem Biophys. 1999 Jul 1;367(1):9-16. DOI:10.1006/abbi.1999.1255 | PubMed ID:10375393 | HubMed [Ross-ArchBiochemBiophys-1999]
  3. Pott MB, Hippauf F, Saschenbrecker S, Chen F, Ross J, Kiefer I, Slusarenko A, Noel JP, Pichersky E, Effmert U, and Piechulla B. Biochemical and structural characterization of benzenoid carboxyl methyltransferases involved in floral scent production in Stephanotis floribunda and Nicotiana suaveolens. Plant Physiol. 2004 Aug;135(4):1946-55. DOI:10.1104/pp.104.041806 | PubMed ID:15310828 | HubMed [Pott-PlantPhysiol-2004]
  4. Zubieta C, Ross JR, Koscheski P, Yang Y, Pichersky E, and Noel JP. Structural basis for substrate recognition in the salicylic acid carboxyl methyltransferase family. Plant Cell. 2003 Aug;15(8):1704-16. DOI:10.1105/tpc.014548 | PubMed ID:12897246 | HubMed [Zubieta-PlantCell-2003]

All Medline abstracts: PubMed | HubMed

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