IGEM:MIT/2006/Communications

Figure 1

• (a) normal e. coli (depict chemical inputs and outputs)
• (b) final eau d’e coli system (depict chemical inputs and outputs)

Figure 1 (pdf)

Figure 2

Device depictions (show one or two devices at the parts level, perhaps also show a device transfer function and also whatever the most relevant device data is, perhaps the GC data)

Figure 2 (pdf)

Figure 3

GC data (banana total synthesis, wintergreen total synthesis, banana with precursor, wintergreen with precursor)

Figure 4

Smell test data (show photo of smell test in action and also bar graphs of results)

Jamboree smell test data (xls)

Bar graph of smell test results (pdf)

Figure 5

Growth phase control (parts and device level depiction)

Figure 5 (pdf)

• verify the part numbers of the devices for which there is GC data ...

J45993 vs J45992 and J45199 vs J45219

Figure 6

Show data with growth-phase control of FP output

• ask Barry to make pretty figure for this data

Figure 7

Show GC data from banana time course

Figure S1

Restrospective project timeline. How long did each step take? [from this we can compute how we could do such work faster. We’ll want to work the pace of work into both the introduction and discussion].

Figure S2

Show GC data here (could have chromatograms)? GC data for IK cells versus normal E. coli; mint scented bacteria versus IK cells.

References

Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."

• Isoamyl acetate
• Methyl salicylate

Use of ATF1 in E. coli

1. Singh R, Vadlani PV, Harrison ML, Bennett GN, and San KY. Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferases AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients. Bioprocess Biosyst Eng. 2008 Jun;31(4):299-306. DOI:10.1007/s00449-007-0159-3 |

Methyl salicylate synthesis

2. Negre F, Kolosova N, Knoll J, Kish CM, and Dudareva N. Novel S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme responsible for biosynthesis of methyl salicylate and methyl benzoate, is not involved in floral scent production in snapdragon flowers. Arch Biochem Biophys. 2002 Oct 15;406(2):261-70. DOI:10.1016/s0003-9861(02)00458-7 |
3. Ross JR, Nam KH, D'Auria JC, and Pichersky E. S-Adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme involved in floral scent production and plant defense, represents a new class of plant methyltransferases. Arch Biochem Biophys. 1999 Jul 1;367(1):9-16. DOI:10.1006/abbi.1999.1255 |
4. Pott MB, Hippauf F, Saschenbrecker S, Chen F, Ross J, Kiefer I, Slusarenko A, Noel JP, Pichersky E, Effmert U, and Piechulla B. Biochemical and structural characterization of benzenoid carboxyl methyltransferases involved in floral scent production in Stephanotis floribunda and Nicotiana suaveolens. Plant Physiol. 2004 Aug;135(4):1946-55. DOI:10.1104/pp.104.041806 |
5. Zubieta C, Ross JR, Koscheski P, Yang Y, Pichersky E, and Noel JP. Structural basis for substrate recognition in the salicylic acid carboxyl methyltransferase family. Plant Cell. 2003 Aug;15(8):1704-16. DOI:10.1105/tpc.014548 |