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Figure 1

Complete system diagram.


Figure 2

Smell generating devices from precursor. Image:MITiGEM2006Figure2.pdf

Figure 3

Autonomous wintergreen production

  • (a) Device and parts level diagram of autonomous wintergreen synthesis
  • (b) GC data of wintergreen total synthesis
  • (c) GC data of J45120 without precursor
  • (d) Pure methyl salicylate standard


Figure 4

Growth phase control

  • (a) parts and device level depiction
  • (b) growth-phase control of FP output


Figure 5

  • (a) device diagram of regulated banana device
  • (b) timecourse of banana smell



Figure S1

Restrospective project timeline. How long did each step take? [from this we can compute how we could do such work faster. We’ll want to work the pace of work into both the introduction and discussion].

Figure S2

  • (a) GC data for IK cells versus normal E. coli;
  • (b) mint scented bacteria with precursor versus TOP10 with precursor.
  • (c) banana scented bacteria with precursor versus TOP10 with precursor.


Also see the relevant part pages for references.

osmY Promoter Mehods Section (In order)

book reference:

Chasel, M. Gentry D.R., Hernandez, V.J., and Vinella, D. (1996) in Neidhardt, F.C. (ed.) Escherichia coli and Solmonella-Cellular and Molecular Biology, 2nd edition. pp. 1476-1477, ASM Press, Washington, DC.

Colland paper:


Schellhorn paper:


Yim et al., reference:


Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."

Use of ATF1 in E. coli

Error fetching PMID 17891501:
  1. Error fetching PMID 17891501: [Singh-BioprocessBiosysEng-2007]

Methyl salicylate synthesis

Error fetching PMID 12361714:
Error fetching PMID 10375393:
Error fetching PMID 15310828:
Error fetching PMID 12897246:
  1. Error fetching PMID 12361714: [Negre-ArchBiochemBiophys-2002]
  2. Error fetching PMID 10375393: [Ross-ArchBiochemBiophys-1999]
  3. Error fetching PMID 15310828: [Pott-PlantPhysiol-2004]
  4. Error fetching PMID 12897246: [Zubieta-PlantCell-2003]
All Medline abstracts: PubMed HubMed
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