IGEM:MIT/2006/Communications

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Contents 1 Figure 1 2 Figure 2 3 Figure 3 4 Figure 4 5 Figure 5 6 Figure S1 7 Figure S2 8 References 8.1 osmY Promoter Mehods Section (In order) 8.2 Odor thresholds 8.3 Use of ATF1 in E. coli 8.4 Methyl salicylate synthesis

Figure 1

Complete system diagram.

Image:MITiGEM2006Figure1.pdf

Figure 2

Smell generating devices from precursor. Image:MITiGEM2006Figure2.pdf

• (a) device diagrams of wintergreen and banana generating devices.
  • Image:MITiGEM2006Figure2a.pdf
• (b) smell test results from Jamboree
  • Image:IGEM06Smelltest.xls (xls)
• (c) smell test photo?

Figure 3

Autonomous wintergreen production

• (a) Device and parts level diagram of autonomous wintergreen synthesis
• (b) GC data of wintergreen total synthesis
• (c) GC data of J45120 without precursor
• (d) Pure methyl salicylate standard

Image:MITiGEM2006Figure3.pdf

Figure 4

Growth phase control

• (a) parts and device level depiction
• (b) growth-phase control of FP output

Image:MITiGEM2006Figure4.pdf

Figure 5

• (a) device diagram of regulated banana device
• (b) timecourse of banana smell

Image:MITiGEM2006Figure5.pdf

Image:MITiGEM2006Figure5b.pdf

Figure S1

Restrospective project timeline. How long did each step take? [from this we can compute how we could do such work faster. We’ll want to work the pace of work into both the introduction and discussion].

Figure S2

• (a) GC data for IK cells versus normal E. coli;
• (b) mint scented bacteria with precursor versus TOP10 with precursor.
• (c) banana scented bacteria with precursor versus TOP10 with precursor.

References

Also see the relevant part pages for references.

osmY Promoter Mehods Section (In order)

book reference:

Chasel, M. Gentry D.R., Hernandez, V.J., and Vinella, D. (1996) in Neidhardt, F.C. (ed.) Escherichia coli and Solmonella-Cellular and Molecular Biology, 2nd edition. pp. 1476-1477, ASM Press, Washington, DC.

Colland paper:

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=203352

Schellhorn paper:

http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829938

Yim et al., reference:

http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8282684

Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."

• Isoamyl acetate
• Methyl salicylate

Use of ATF1 in E. coli

1. Singh R, Vadlani PV, Harrison ML, Bennett GN, and San KY. . pmid:17891501. PubMed HubMed [Singh-BioprocessBiosysEng-2007]

Methyl salicylate synthesis

2. Negre F, Kolosova N, Knoll J, Kish CM, and Dudareva N. . pmid:12361714. PubMed HubMed [Negre-ArchBiochemBiophys-2002]
3. Ross JR, Nam KH, D'Auria JC, and Pichersky E. . pmid:10375393. PubMed HubMed [Ross-ArchBiochemBiophys-1999]
4. Pott MB, Hippauf F, Saschenbrecker S, Chen F, Ross J, Kiefer I, Slusarenko A, Noel JP, Pichersky E, Effmert U, and Piechulla B. . pmid:15310828. PubMed HubMed [Pott-PlantPhysiol-2004]
5. Zubieta C, Ross JR, Koscheski P, Yang Y, Pichersky E, and Noel JP. . pmid:12897246. PubMed HubMed [Zubieta-PlantCell-2003]

All Medline abstracts: PubMed HubMed