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Figure 1

Complete system diagram.


Figure 2

Smell generating devices from precursor. Image:MITiGEM2006Figure2.pdf

Figure 3

Autonomous wintergreen production

  • (a) Device and parts level diagram of autonomous wintergreen synthesis
  • (b) GC data of wintergreen total synthesis
  • (c) GC data of J45120 without precursor
  • (d) Pure methyl salicylate standard


Figure 4

Growth phase control

  • (a) parts and device level depiction
  • (b) growth-phase control of FP output


Figure 5

  • (a) device diagram of regulated banana device
  • (b) timecourse of banana smell



Figure S1

Restrospective project timeline. How long did each step take? [from this we can compute how we could do such work faster. We’ll want to work the pace of work into both the introduction and discussion].

Figure S2

  • (a) GC data for IK cells versus normal E. coli;
  • (b) mint scented bacteria with precursor versus TOP10 with precursor.
  • (c) banana scented bacteria with precursor versus TOP10 with precursor.


Also see the relevant part pages for references.

osmY Promoter Mehods Section (In order)

book reference:

Chasel, M. Gentry D.R., Hernandez, V.J., and Vinella, D. (1996) in Neidhardt, F.C. (ed.) Escherichia coli and Salmonella-Cellular and Molecular Biology, 2nd edition. pp. 1476-1477, ASM Press, Washington, DC.

  1. editors, Frederick C. Neidhardt... [et al.]. Escherichia coli and Salmonella. Washington, D.C.: ASM Press, 1996. isbn:1555810845. [Neidhardt]
  2. Colland F, Barth M, Hengge-Aronis R, and Kolb A. . pmid:10856246. PubMed HubMed [Colland-EMBO-2000]
  3. Schellhorn HE, Audia JP, Wei LI, and Chang L. . pmid:9829938. PubMed HubMed [Schelhorn]
  4. Yim HH, Brems RL, and Villarejo M. . pmid:8282684. PubMed HubMed [Yim]
All Medline abstracts: PubMed HubMed

Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."

Use of ATF1 in E. coli

  1. Singh R, Vadlani PV, Harrison ML, Bennett GN, and San KY. . pmid:17891501. PubMed HubMed [Singh-BioprocessBiosysEng-2007]

Methyl salicylate synthesis

  1. Negre F, Kolosova N, Knoll J, Kish CM, and Dudareva N. . pmid:12361714. PubMed HubMed [Negre-ArchBiochemBiophys-2002]
  2. Ross JR, Nam KH, D'Auria JC, and Pichersky E. . pmid:10375393. PubMed HubMed [Ross-ArchBiochemBiophys-1999]
  3. Pott MB, Hippauf F, Saschenbrecker S, Chen F, Ross J, Kiefer I, Slusarenko A, Noel JP, Pichersky E, Effmert U, and Piechulla B. . pmid:15310828. PubMed HubMed [Pott-PlantPhysiol-2004]
  4. Zubieta C, Ross JR, Koscheski P, Yang Y, Pichersky E, and Noel JP. . pmid:12897246. PubMed HubMed [Zubieta-PlantCell-2003]
All Medline abstracts: PubMed HubMed
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