IGEM:MIT/2006/Index: Difference between revisions
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#*Explore biosynthesis of precursors | #*Explore biosynthesis of precursors | ||
#*Sequence BAMT, SAMT, BSMT | #*Sequence BAMT, SAMT, BSMT | ||
#**From initial Top10 with | #**From initial Top10 with [[../Notebook/2006-6-13#pET-28 Primers |T7/TPhiR]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]] | ||
#** | #**Troubleshoot SAMT with [[../Notebook/2006-6-8#SAMT from A.Majus |designed reverse primer]] | ||
#Expression in E. coli | #Expression in E. coli | ||
#*Transformation into Top10 | #*Transformation into Top10 | ||
Revision as of 12:22, 16 June 2006
Projects/Progress
- Preliminary Research
- Determine toxicity of precursors and products (VV)
- [[../Notebook/2006-6-16#Toxicity |6-16-2006]]
- Test effects of minimal media on smell
- Determine the origin of the bad smell (indole?)
- Explore biosynthesis of precursors
- Sequence BAMT, SAMT, BSMT
- From initial Top10 with [[../Notebook/2006-6-13#pET-28 Primers |T7/TPhiR]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
- Troubleshoot SAMT with [[../Notebook/2006-6-8#SAMT from A.Majus |designed reverse primer]]
- Determine toxicity of precursors and products (VV)
- Expression in E. coli
- Transformation into Top10
- Transformation into BL21 (DE3)
- [[../Notebook/2006-6-16#BL21 Tranformation |6-16-2006]]
- Applications
- Heat promoter
- Yeast incorporation
- Quantification
- Look up GC use
