IGEM:MIT/2006/Index

Projects/Progress

1. Preliminary Research
   • Determine toxicity of precursors and products (VV)
     • [[../Notebook/2006-6-16#Toxicity |6-16-2006]]
   • Test effects of minimal media on smell
   • Determine the origin of the bad smell (indole?)
   • Explore biosynthesis of precursors
   • Sequence BAMT, SAMT, BSMT
     • From initial Top10 with [[../Notebook/2006-6-13#pET-28 Primers |T7/TPhiR]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
     • Troubleshoot SAMT with [[../Notebook/2006-6-8#SAMT from A.Majus |designed reverse primer]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
2. Expression in E. coli
   • Transformation into Top10
   • Transformation into BL21 (DE3)
     • [[../Notebook/2006-6-16#BL21 Tranformation |6-16-2006]]
3. Applications
   • Heat promoter
4. Yeast incorporation
   • Discussion at [[../Notebook/2006-6-19#Yeast_Incorporation |6-19-2006]]
5. Quantification
   • Look up GC use
     • Discussion at [[../Notebook/2006-6-14#Resources_on_gas_chromatography-olfactometry |6-14-06]]