IGEM:MIT/2006/Index: Difference between revisions

Revision as of 11:46, 20 June 2006

Preliminary Research
- Determine toxicity of precursors and products (VV) (complete)
  - [[../Notebook/2006-6-16#Toxicity |6-16-2006]] covered toxicity
- Test effects of minimal media on smell
  - Reduced smell with no tryptophan, [[../Notebook/2006-6-20#WINTERGREEN!!!!!!! |6-20-2006]] - now testing other mediums to find best
- Determine the origin of the bad smell (indole?) (complete)
  - Indole is bad smell, [[../Notebook/2006-6-20#Indole |6-20-2006]] - now looking to get knockouts
- Explore biosynthesis of precursors
- Sequence BAMT, SAMT, BSMT
  - From initial Top10 with [[../Notebook/2006-6-13#pET-28 Primers |T7/TPhiR]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
  - Troubleshoot SAMT with [[../Notebook/2006-6-8#SAMT from A.Majus |designed reverse primer]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]

#Expression in E. coli

- Transformation into Top10 (complete)
- Transformation into BL21 (DE3)
  - [[../Notebook/2006-6-16#BL21 Tranformation |6-16-2006]]
- Indole knockout

#Applications

- Heat promoter
Yeast incorporation
- Discussion at [[../Notebook/2006-6-19#Yeast_Incorporation |6-19-2006]]
- Preliminary experiments with ATF1 [[../Notebook/2006-6-15 |6-15-2006]] and [[../Notebook/2006-6-19#Repeat ATF1 PCR |6-19-2006]]

#Quantification

- Look up GC use
  - Discussion at [[../Notebook/2006-6-14#Resources_on_gas_chromatography-olfactometry |6-14-06]]
  - Documentation on the appropriate instrumentation and procedure for GC with methyl benzoate here

@@ Line 1: / Line 1: @@
 ==Projects/Progress==
-#Preliminary Research
+#<b>Preliminary Research</b>
 #*Determine toxicity of precursors and products (VV) <b>(complete)</b>
 #**[[../Notebook/2006-6-16#Toxicity |6-16-2006]] covered toxicity
@@ Line 11: / Line 11: @@
 #**From initial Top10 with [[../Notebook/2006-6-13#pET-28 Primers |T7/TPhiR]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
 #**Troubleshoot SAMT with [[../Notebook/2006-6-8#SAMT from A.Majus |designed reverse primer]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
-#Expression in E. coli
+<b>#Expression in E. coli</b>
-#*Transformation into Top10
+#*Transformation into Top10 <b>(complete)</b>
 #*Transformation into BL21 (DE3)
 #**[[../Notebook/2006-6-16#BL21 Tranformation |6-16-2006]]
-#Applications
+#*Indole knockout
+<b>#Applications</b>
 #*Heat promoter
 #Yeast incorporation
 #*Discussion at [[../Notebook/2006-6-19#Yeast_Incorporation |6-19-2006]]
 #*Preliminary experiments with ATF1 [[../Notebook/2006-6-15 |6-15-2006]] and [[../Notebook/2006-6-19#Repeat ATF1 PCR |6-19-2006]]
-#Quantification
+<b>#Quantification</b>
 #*Look up GC use
 #**Discussion at [[../Notebook/2006-6-14#Resources_on_gas_chromatography-olfactometry |6-14-06]]
 #**Documentation on the appropriate instrumentation and procedure for GC with methyl benzoate [http://web.mit.edu/boyuan/Public/J_Sep_Sci_2005_mold.pdf here]