IGEM:MIT/2006/Index: Difference between revisions
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#*Determine toxicity of precursors and products (VV) <b>(complete)</b> | #*Determine toxicity of precursors and products (VV) <b>(complete)</b> | ||
#**[[../Notebook/2006-6-16#Toxicity |6-16-2006]] covered toxicity | #**[[../Notebook/2006-6-16#Toxicity |6-16-2006]] covered toxicity | ||
#*Test effects of minimal media on smell | #*Test effects of minimal media on smell <b>(complete)</b> | ||
#**Reduced smell with no tryptophan, [[../Notebook/2006-6-20#WINTERGREEN!!!!!!! |6-20-2006]] - now testing other mediums to find best | #**Reduced smell with no tryptophan, [[../Notebook/2006-6-20#WINTERGREEN!!!!!!! |6-20-2006]] - now testing other mediums to find best | ||
#**Determine optimal concentration of SA: 400 uL | |||
#*Determine the origin of the bad smell (indole?) <b>(complete)</b> | #*Determine the origin of the bad smell (indole?) <b>(complete)</b> | ||
#**Indole is bad smell, [[../Notebook/2006-6-20#Indole |6-20-2006]] - now looking to get knockouts | #**Indole is bad smell, [[../Notebook/2006-6-20#Indole |6-20-2006]] - now looking to get knockouts |
Revision as of 10:52, 27 June 2006
Projects/Progress
- Preliminary Research
- Determine toxicity of precursors and products (VV) (complete)
- [[../Notebook/2006-6-16#Toxicity |6-16-2006]] covered toxicity
- Test effects of minimal media on smell (complete)
- Reduced smell with no tryptophan, [[../Notebook/2006-6-20#WINTERGREEN!!!!!!! |6-20-2006]] - now testing other mediums to find best
- Determine optimal concentration of SA: 400 uL
- Determine the origin of the bad smell (indole?) (complete)
- Indole is bad smell, [[../Notebook/2006-6-20#Indole |6-20-2006]] - now looking to get knockouts
- Explore biosynthesis of precursors
- Sequence BAMT, SAMT, BSMT
- From initial Top10 with [[../Notebook/2006-6-13#pET-28 Primers |T7/TPhiR]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
- Troubleshoot SAMT with [[../Notebook/2006-6-8#SAMT from A.Majus |designed reverse primer]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
- Determine toxicity of precursors and products (VV) (complete)
- Expression in E. coli
- Transformation into Top10 (complete)
- Transformation into BL21 (DE3)
- [[../Notebook/2006-6-16#BL21 Tranformation |6-16-2006]]
- Indole knockout
- Applications
- Heat promoter
- Yeast incorporation
- Discussion at [[../Notebook/2006-6-19#Yeast_Incorporation |6-19-2006]]
- Preliminary experiments with ATF1 [[../Notebook/2006-6-15 |6-15-2006]] and [[../Notebook/2006-6-19#Repeat ATF1 PCR |6-19-2006]]
- Quantification
- Look up GC use
- Discussion at [[../Notebook/2006-6-14#Resources_on_gas_chromatography-olfactometry |6-14-06]]
- Documentation on the appropriate instrumentation and procedure for GC with methyl benzoate here
- Look up GC use