IGEM:MIT/2006/Index: Difference between revisions
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#*Preliminary experiments with ATF1 [[../Notebook/2006-6-15 |6-15-2006]] and [[../Notebook/2006-6-19#Repeat ATF1 PCR |6-19-2006]] | #*Preliminary experiments with ATF1 [[../Notebook/2006-6-15 |6-15-2006]] and [[../Notebook/2006-6-19#Repeat ATF1 PCR |6-19-2006]] | ||
#<b>Quantification</b> | #<b>Quantification</b> | ||
#* | #*Get trained for GC/MS use | ||
#**Discussion at [[../Notebook/2006-6-14#Resources_on_gas_chromatography-olfactometry |6-14-06]] | #**Discussion at [[../Notebook/2006-6-14#Resources_on_gas_chromatography-olfactometry |6-14-06]] | ||
#**Documentation on the appropriate instrumentation and procedure for GC with methyl benzoate [http://web.mit.edu/boyuan/Public/J_Sep_Sci_2005_mold.pdf here] | #**Documentation on the appropriate instrumentation and procedure for GC with methyl benzoate [http://web.mit.edu/boyuan/Public/J_Sep_Sci_2005_mold.pdf here] | ||
==Protocols== | |||
Revision as of 10:53, 27 June 2006
Projects/Progress
- Preliminary Research
- Determine toxicity of precursors and products (VV) (complete)
- [[../Notebook/2006-6-16#Toxicity |6-16-2006]] covered toxicity
- Test effects of minimal media on smell (complete)
- Reduced smell with no tryptophan, [[../Notebook/2006-6-20#WINTERGREEN!!!!!!! |6-20-2006]] - now testing other mediums to find best
- Determine optimal concentration of SA: 400 uL
- Determine the origin of the bad smell (indole?) (complete)
- Indole is bad smell, [[../Notebook/2006-6-20#Indole |6-20-2006]] - now looking to get knockouts
- Explore biosynthesis of precursors
- Sequence BAMT, SAMT, BSMT
- From initial Top10 with [[../Notebook/2006-6-13#pET-28 Primers |T7/TPhiR]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
- Troubleshoot SAMT with [[../Notebook/2006-6-8#SAMT from A.Majus |designed reverse primer]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
- Determine toxicity of precursors and products (VV) (complete)
- Expression in E. coli
- Transformation into Top10 (complete)
- Transformation into BL21 (DE3)
- [[../Notebook/2006-6-16#BL21 Tranformation |6-16-2006]]
- Indole knockout
- Applications
- Heat promoter
- Yeast incorporation
- Discussion at [[../Notebook/2006-6-19#Yeast_Incorporation |6-19-2006]]
- Preliminary experiments with ATF1 [[../Notebook/2006-6-15 |6-15-2006]] and [[../Notebook/2006-6-19#Repeat ATF1 PCR |6-19-2006]]
- Quantification
- Get trained for GC/MS use
- Discussion at [[../Notebook/2006-6-14#Resources_on_gas_chromatography-olfactometry |6-14-06]]
- Documentation on the appropriate instrumentation and procedure for GC with methyl benzoate here
- Get trained for GC/MS use
