IGEM:MIT/2006/Index

Projects/Progress

1. Preliminary Research
   • Determine toxicity of precursors and products (VV) (complete)
     • [[../Notebook/2006-6-16#Toxicity |6-16-2006]] covered toxicity
   • Test effects of minimal media on smell (complete)
     • Reduced smell with no tryptophan, [[../Notebook/2006-6-20#WINTERGREEN!!!!!!! |6-20-2006]] - now testing other mediums to find best
     • Determine optimal concentration of SA: 400 uL
   • Determine the origin of the bad smell (indole?) (complete)
     • Indole is bad smell, [[../Notebook/2006-6-20#Indole |6-20-2006]] - now looking to get knockouts
   • Explore biosynthesis of precursors
   • Sequence BAMT, SAMT, BSMT
     • From initial Top10 with [[../Notebook/2006-6-13#pET-28 Primers |T7/TPhiR]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
     • Troubleshoot SAMT with [[../Notebook/2006-6-8#SAMT from A.Majus |designed reverse primer]] on [[../Notebook/2006-6-16#Sequencing |6-16-2006]]
2. Expression in E. coli
   • Transformation into Top10 (complete)
   • Transformation into BL21 (DE3)
     • [[../Notebook/2006-6-16#BL21 Tranformation |6-16-2006]]
   • Indole knockout
3. Applications
   • Heat promoter
4. Yeast incorporation
   • Discussion at [[../Notebook/2006-6-19#Yeast_Incorporation |6-19-2006]]
   • Preliminary experiments with ATF1 [[../Notebook/2006-6-15 |6-15-2006]] and [[../Notebook/2006-6-19#Repeat ATF1 PCR |6-19-2006]]
5. Quantification
   • Get trained for GC/MS use
     • Discussion at [[../Notebook/2006-6-14#Resources_on_gas_chromatography-olfactometry |6-14-06]]
     • Documentation on the appropriate instrumentation and procedure for GC with methyl benzoate here

Protocols

1. PCR
2. Digestion [[../Notebook/2006-6-27#Restriction Digest |6-27-2006]]
3. Ligation [[../Notebook/2006-6-21#Ligation reaction |6-21-2006]] Knight
4. Transformation Transform competent cells