IGEM:MIT/2006/Introduction: Difference between revisions
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To complete your online safety training, visit the [[Endy:Safety_Training | '''Endy lab safety training guide''']]. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) | To complete your online safety training, visit the [[Endy:Safety_Training | '''Endy lab safety training guide''']]. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) | ||
This should be done before your first day in lab. | |||
Heather will be giving the lab-specific training | Heather will be giving the lab-specific training <font color=red>- Day 1, Week 1</font> | ||
==Wiki== | ==Wiki== | ||
'''How-to on wiki's''' | '''How-to on wiki's''' | ||
*[[OpenWetWare:Getting started | Getting started]] - | *[[OpenWetWare:Getting started | Getting started with wiki editing]] <font color=red>- Day 1, Week 1</font> | ||
*Populate the official iGEM wiki | *Populate the official iGEM wiki | ||
| Line 18: | Line 19: | ||
==Registry== | ==Registry== | ||
*Obtain accounts | *Obtain accounts <font color=red>- Day 1, Week 1</font> | ||
*Making a part (both basic and subpart) | *Making a part (both basic and subpart) <font color=red>- Day 1, Week 1</font> | ||
*BLAST | *BLAST | ||
*Sequence alignments | *Sequence alignments | ||
==BioBricks assembly== | ==BioBricks assembly== | ||
*[[BioBricks]] | *[[BioBricks]] <font color=red>- Day 1, Week 1</font> | ||
*Standard assembly | *Standard assembly <font color=blue>- Day 2, Week 1</font> | ||
*3A assembly | *3A assembly | ||
*In frame assembly | *In frame assembly | ||
Revision as of 16:59, 25 May 2006
This page contains links to introductory information for the MIT iGEM team.
Lab safety
All people working in a biological research lab must undergo safety training.
To complete your online safety training, visit the Endy lab safety training guide. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab.
Heather will be giving the lab-specific training - Day 1, Week 1
Wiki
How-to on wiki's
- Getting started with wiki editing - Day 1, Week 1
- Populate the official iGEM wiki
Abstraction hierarchy
- PoPS and composability
Registry
- Obtain accounts - Day 1, Week 1
- Making a part (both basic and subpart) - Day 1, Week 1
- BLAST
- Sequence alignments
BioBricks assembly
- BioBricks - Day 1, Week 1
- Standard assembly - Day 2, Week 1
- 3A assembly
- In frame assembly
- Direct synthesis
Part and device characterization
- Plate reader characterization (i.e. Receiver)
- Flow cytometry (i.e. Screening plasmid)
- Microscopy
- RT-PCR and Westerns
Searching and reading the literature
Lab techniques
Links
General introduction to ideas in Synthetic Biology
- Adventures in synthetic biology comic --> Learn about PoPS
- Foundations for engineering biology --> rant by Drew Endy
- MIT iGEM 2004 presentation (.ppt,pdf)
- UT Austin and UCSF 2004 project (coliroids)
- Find descriptions of 2005 projects
Week 1
Goals -
- Do one assembly stage
- Make a biobrick
- Necessary software - VectorNTI, Registry, Wiki
- Literature searching
Day 1
Dry Lab
- Safety training
- Lab tour
- Registry accounts
Wet Lab
- Pick a gene and design primers
- Introduces parts
- Start overnights
- Introduces sterile technique, culturing,
Day 2
Dry Lab
- BioBricks Construction
Wet Lab
- Prep & Digest
