IGEM:MIT/2006/Introduction: Difference between revisions
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This page contains links to introductory information for the MIT iGEM team. | This page contains links to introductory information for the MIT iGEM team. | ||
== | ==Design== | ||
===System Design considerations=== | |||
*Feasible system scale (numbers of parts, plasmids, chassis types) | |||
*Design tools - models, network diagrams | |||
*Moving from system->devices->parts->DNA specification | |||
*Likelihood of success - protein engineering, cloning, new biological techniques and new science etc. | |||
===Abstraction hierarchy & standardization=== | |||
==Abstraction hierarchy & standardization== | |||
*Parts - zinc fingers? | *Parts - zinc fingers? | ||
*Devices - receiver?, inverter? | *Devices - receiver?, inverter? | ||
| Line 21: | Line 14: | ||
*PoPS and composability | *PoPS and composability | ||
== | ==Build== | ||
===BioBricks assembly=== | |||
==BioBricks assembly== | |||
*[[BioBricks]] <font color=red>- Day 1, Week 1</font> | *[[BioBricks]] <font color=red>- Day 1, Week 1</font> | ||
*Standard assembly <font color=blue>- Day 2, Week 1</font> | *Standard assembly <font color=blue>- Day 2, Week 1</font> | ||
| Line 34: | Line 22: | ||
*Direct synthesis | *Direct synthesis | ||
==Part and device characterization== | ===Registry=== | ||
*Obtain accounts <font color=red>- Day 1, Week 1</font> | |||
*Making a part (both basic and subpart) <font color=red>- Day 1, Week 1</font> | |||
*BLAST | |||
*Sequence alignments | |||
==Test== | |||
===Part and device characterization=== | |||
*Plate reader characterization (i.e. Receiver) | *Plate reader characterization (i.e. Receiver) | ||
*Flow cytometry (i.e. Screening plasmid) | *Flow cytometry (i.e. Screening plasmid) | ||
| Line 40: | Line 35: | ||
*RT-PCR and Westerns | *RT-PCR and Westerns | ||
==Searching and reading the literature== | ==Necessary skills== | ||
===Lab notebooks & documentation=== | |||
'''How-to on wiki's'''<br> | |||
*[[OpenWetWare:Getting started | Getting started with wiki editing]] <font color=red>- Day 1, Week 1</font> | |||
*Populate the official iGEM wiki | |||
===Searching and reading the literature=== | |||
*[[Searching the literature]] <font color=green>- Day 3, Week 1</font> | *[[Searching the literature]] <font color=green>- Day 3, Week 1</font> | ||
==Lab | ===Lab safety certification=== | ||
To complete your online safety training, visit the [[Endy:Safety_Training | '''Endy lab safety training guide''']]. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab. | |||
Heather will be giving the lab-specific training <font color=red>- Day 1, Week 1</font> | |||
==Links== | ==Links== | ||
'''General introduction to ideas in Synthetic Biology''' | '''General introduction to ideas in Synthetic Biology''' | ||
#[[Adventures | Adventures in synthetic biology comic]] --> Learn about PoPS | #[[Adventures | Adventures in synthetic biology comic]] --> Learn about PoPS | ||
#[http://openwetware.org/images/0/0d/Nature04342.pdf Foundations for engineering biology] --> rant by Drew Endy | #[http://openwetware.org/images/0/0d/Nature04342.pdf Foundations for engineering biology] --> rant by Drew Endy | ||
| Line 54: | Line 57: | ||
#Find descriptions of [http://parts2.mit.edu/wiki/index.php/Igem_2005 2005 projects] | #Find descriptions of [http://parts2.mit.edu/wiki/index.php/Igem_2005 2005 projects] | ||
== | ==Schedules== | ||
*[[IGEM:MIT/2006/Introduction/Week 1]] | |||
* | |||
Revision as of 17:21, 25 May 2006
This page contains links to introductory information for the MIT iGEM team.
Design
System Design considerations
- Feasible system scale (numbers of parts, plasmids, chassis types)
- Design tools - models, network diagrams
- Moving from system->devices->parts->DNA specification
- Likelihood of success - protein engineering, cloning, new biological techniques and new science etc.
Abstraction hierarchy & standardization
- Parts - zinc fingers?
- Devices - receiver?, inverter?
- Systems - bacterial photography?
- PoPS and composability
Build
BioBricks assembly
- BioBricks - Day 1, Week 1
- Standard assembly - Day 2, Week 1
- 3A assembly
- In frame assembly
- Direct synthesis
Registry
- Obtain accounts - Day 1, Week 1
- Making a part (both basic and subpart) - Day 1, Week 1
- BLAST
- Sequence alignments
Test
Part and device characterization
- Plate reader characterization (i.e. Receiver)
- Flow cytometry (i.e. Screening plasmid)
- Microscopy
- RT-PCR and Westerns
Necessary skills
Lab notebooks & documentation
How-to on wiki's
- Getting started with wiki editing - Day 1, Week 1
- Populate the official iGEM wiki
Searching and reading the literature
- Searching the literature - Day 3, Week 1
Lab safety certification
To complete your online safety training, visit the Endy lab safety training guide. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab.
Heather will be giving the lab-specific training - Day 1, Week 1
Links
General introduction to ideas in Synthetic Biology
- Adventures in synthetic biology comic --> Learn about PoPS
- Foundations for engineering biology --> rant by Drew Endy
- MIT iGEM 2004 presentation (.ppt,pdf)
- UT Austin and UCSF 2004 project (coliroids)
- Find descriptions of 2005 projects
