IGEM:MIT/2006/Introduction
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This page contains links to introductory information for the MIT iGEM team. | This page contains links to introductory information for the MIT iGEM team. | ||
| - | == | + | ==Design== |
| - | + | ===System Design considerations=== | |
| + | *Feasible system scale (numbers of parts, plasmids, chassis types) | ||
| + | *Design tools - models, network diagrams | ||
| + | *Moving from system->devices->parts->DNA specification | ||
| + | *Likelihood of success - protein engineering, cloning, new biological techniques and new science etc. | ||
| - | + | ===Abstraction hierarchy & standardization=== | |
| - | + | *Parts - zinc fingers? | |
| - | + | *Devices - receiver?, inverter? | |
| - | + | *Systems - bacterial photography? | |
| + | *PoPS and composability | ||
| - | == | + | ==Build== |
| - | + | ===Construction methods=== | |
| + | *[[BioBricks]] <font color=red>- Day 1, Week 1</font> | ||
| + | *Standard assembly <font color=blue>- Day 2, Week 1</font> | ||
| + | *3A assembly | ||
| + | *In frame assembly | ||
| + | *Direct synthesis | ||
| - | #[[OpenWetWare:Getting started | Getting started]] --> | + | ===Construction tools=== |
| + | *Registry | ||
| + | *#Obtain accounts <font color=red>- Day 1, Week 1</font> | ||
| + | *#Making a part (both basic and composite) <font color=red>- Day 1, Week 1</font> | ||
| + | *#BLAST | ||
| + | *#Sequence alignments | ||
| + | *NCBI Entrez <font color=red>- Day 1, Week 1</font> | ||
| + | *VectorNTI | ||
| + | *Others? | ||
| + | |||
| + | ==Test== | ||
| + | *Plate reader characterization (i.e. Receiver) | ||
| + | *Flow cytometry (i.e. Screening plasmid) | ||
| + | *Microscopy | ||
| + | *RT-PCR and Westerns | ||
| + | |||
| + | ==Necessary skills== | ||
| + | ===Lab notebooks & documentation=== | ||
| + | *[[OpenWetWare:Getting started | Getting started with wiki editing]] <font color=red>- Day 1, Week 1</font> | ||
| + | *Populate the [http://parts.mit.edu/wiki/index.php/MIT_2006 official MIT iGEM 2006 wiki page] | ||
| + | *The online lab notebook framework | ||
| + | |||
| + | ===Searching and reading the literature=== | ||
| + | *[[Searching the literature]] <font color=green>- Day 3, Week 1</font> | ||
| + | |||
| + | ===Lab safety=== | ||
| + | *Complete the [[Endy:Safety_Training | '''Endy lab safety training''']]. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab. | ||
| + | *Heather will be giving the lab-specific training <font color=red>- Day 1, Week 1</font> | ||
| + | |||
| + | ==Links== | ||
| + | '''General introduction to ideas in Synthetic Biology''' | ||
| + | #[[Adventures | Adventures in synthetic biology comic]] --> Learn about PoPS | ||
| + | #[http://openwetware.org/images/0/0d/Nature04342.pdf Foundations for engineering biology] --> rant by Drew Endy | ||
| + | #MIT iGEM 2004 presentation ([[:Image:Mit-2004-iGEM-final.ppt|.ppt]],[[:Image:Mit-iGEM-2004-final.pdf|pdf]]) | ||
| + | #UT Austin and UCSF 2004 project ([http://parts.mit.edu/registry/index.php/Featured_Parts:Light_Sensor coliroids]) | ||
| + | #Find descriptions of [http://parts2.mit.edu/wiki/index.php/Igem_2005 2005 projects] | ||
| - | |||
| - | |||
| - | + | ==Schedules== | |
| + | *[[IGEM:MIT/2006/Introduction/Week 1]] | ||
Current revision
This page contains links to introductory information for the MIT iGEM team.
Contents |
Design
System Design considerations
- Feasible system scale (numbers of parts, plasmids, chassis types)
- Design tools - models, network diagrams
- Moving from system->devices->parts->DNA specification
- Likelihood of success - protein engineering, cloning, new biological techniques and new science etc.
Abstraction hierarchy & standardization
- Parts - zinc fingers?
- Devices - receiver?, inverter?
- Systems - bacterial photography?
- PoPS and composability
Build
Construction methods
- BioBricks - Day 1, Week 1
- Standard assembly - Day 2, Week 1
- 3A assembly
- In frame assembly
- Direct synthesis
Construction tools
- Registry
- Obtain accounts - Day 1, Week 1
- Making a part (both basic and composite) - Day 1, Week 1
- BLAST
- Sequence alignments
- NCBI Entrez - Day 1, Week 1
- VectorNTI
- Others?
Test
- Plate reader characterization (i.e. Receiver)
- Flow cytometry (i.e. Screening plasmid)
- Microscopy
- RT-PCR and Westerns
Necessary skills
Lab notebooks & documentation
- Getting started with wiki editing - Day 1, Week 1
- Populate the official MIT iGEM 2006 wiki page
- The online lab notebook framework
Searching and reading the literature
- Searching the literature - Day 3, Week 1
Lab safety
- Complete the Endy lab safety training. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab.
- Heather will be giving the lab-specific training - Day 1, Week 1
Links
General introduction to ideas in Synthetic Biology
- Adventures in synthetic biology comic --> Learn about PoPS
- Foundations for engineering biology --> rant by Drew Endy
- MIT iGEM 2004 presentation (.ppt,pdf)
- UT Austin and UCSF 2004 project (coliroids)
- Find descriptions of 2005 projects
