IGEM:MIT/2006/Introduction

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Current revision (15:14, 2 June 2006) (view source)
(Construction tools)
 
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==Build==
==Build==
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===BioBricks assembly===
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===Construction methods===
*[[BioBricks]] <font color=red>- Day 1, Week 1</font>
*[[BioBricks]] <font color=red>- Day 1, Week 1</font>
*Standard assembly <font color=blue>- Day 2, Week 1</font>
*Standard assembly <font color=blue>- Day 2, Week 1</font>
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*Direct synthesis
*Direct synthesis
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===Registry===
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===Construction tools===
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*Obtain accounts <font color=red>- Day 1, Week 1</font>
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*Registry
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*Making a part (both basic and subpart) <font color=red>- Day 1, Week 1</font>
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*#Obtain accounts <font color=red>- Day 1, Week 1</font>
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*BLAST
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*#Making a part (both basic and composite) <font color=red>- Day 1, Week 1</font>
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*Sequence alignments
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*#BLAST
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*#Sequence alignments
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*NCBI Entrez  <font color=red>- Day 1, Week 1</font>
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*VectorNTI
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*Others?
==Test==
==Test==
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===Part and device characterization===
 
*Plate reader characterization (i.e. Receiver)
*Plate reader characterization (i.e. Receiver)
*Flow cytometry (i.e. Screening plasmid)
*Flow cytometry (i.e. Screening plasmid)
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==Necessary skills==
==Necessary skills==
===Lab notebooks & documentation===
===Lab notebooks & documentation===
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'''How-to on wiki's'''<br>
 
*[[OpenWetWare:Getting started | Getting started with wiki editing]] <font color=red>- Day 1, Week 1</font>
*[[OpenWetWare:Getting started | Getting started with wiki editing]] <font color=red>- Day 1, Week 1</font>
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*Populate the official iGEM wiki
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*Populate the [http://parts.mit.edu/wiki/index.php/MIT_2006 official MIT iGEM 2006 wiki page]
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*The online lab notebook framework
===Searching and reading the literature===
===Searching and reading the literature===
*[[Searching the literature]] <font color=green>- Day 3, Week 1</font>
*[[Searching the literature]] <font color=green>- Day 3, Week 1</font>
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===Lab safety certification===
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===Lab safety===
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To complete your online safety training, visit the [[Endy:Safety_Training | '''Endy lab safety training guide''']].  (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.)  This should be done before your first day in lab.
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*Complete the [[Endy:Safety_Training | '''Endy lab safety training''']].  (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.)  This should be done before your first day in lab.
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*Heather will be giving the lab-specific training <font color=red>- Day 1, Week 1</font>
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Heather will be giving the lab-specific training <font color=red>- Day 1, Week 1</font>
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==Links==
==Links==

Current revision

This page contains links to introductory information for the MIT iGEM team.

Contents

Design

System Design considerations

  • Feasible system scale (numbers of parts, plasmids, chassis types)
  • Design tools - models, network diagrams
  • Moving from system->devices->parts->DNA specification
  • Likelihood of success - protein engineering, cloning, new biological techniques and new science etc.

Abstraction hierarchy & standardization

  • Parts - zinc fingers?
  • Devices - receiver?, inverter?
  • Systems - bacterial photography?
  • PoPS and composability

Build

Construction methods

  • BioBricks - Day 1, Week 1
  • Standard assembly - Day 2, Week 1
  • 3A assembly
  • In frame assembly
  • Direct synthesis

Construction tools

  • Registry
    1. Obtain accounts - Day 1, Week 1
    2. Making a part (both basic and composite) - Day 1, Week 1
    3. BLAST
    4. Sequence alignments
  • NCBI Entrez - Day 1, Week 1
  • VectorNTI
  • Others?

Test

  • Plate reader characterization (i.e. Receiver)
  • Flow cytometry (i.e. Screening plasmid)
  • Microscopy
  • RT-PCR and Westerns

Necessary skills

Lab notebooks & documentation

Searching and reading the literature

Lab safety

  • Complete the Endy lab safety training. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab.
  • Heather will be giving the lab-specific training - Day 1, Week 1

Links

General introduction to ideas in Synthetic Biology

  1. Adventures in synthetic biology comic --> Learn about PoPS
  2. Foundations for engineering biology --> rant by Drew Endy
  3. MIT iGEM 2004 presentation (.ppt,pdf)
  4. UT Austin and UCSF 2004 project (coliroids)
  5. Find descriptions of 2005 projects


Schedules

Personal tools