This page contains links to introductory information for the MIT iGEM team.
System Design considerations
- Feasible system scale (numbers of parts, plasmids, chassis types)
- Design tools - models, network diagrams
- Moving from system->devices->parts->DNA specification
- Likelihood of success - protein engineering, cloning, new biological techniques and new science etc.
Abstraction hierarchy & standardization
- Parts - zinc fingers?
- Devices - receiver?, inverter?
- Systems - bacterial photography?
- PoPS and composability
- BioBricks - Day 1, Week 1
- Standard assembly - Day 2, Week 1
- 3A assembly
- In frame assembly
- Direct synthesis
- Obtain accounts - Day 1, Week 1
- Making a part (both basic and composite) - Day 1, Week 1
- Sequence alignments
- NCBI Entrez
- Plate reader characterization (i.e. Receiver)
- Flow cytometry (i.e. Screening plasmid)
- RT-PCR and Westerns
Lab notebooks & documentation
- Getting started with wiki editing - Day 1, Week 1
- Populate the official MIT iGEM 2006 wiki page
- The online lab notebook framework
Searching and reading the literature
- Searching the literature - Day 3, Week 1
- Complete the Endy lab safety training. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab.
- Heather will be giving the lab-specific training - Day 1, Week 1
General introduction to ideas in Synthetic Biology
- Adventures in synthetic biology comic --> Learn about PoPS
- Foundations for engineering biology --> rant by Drew Endy
- MIT iGEM 2004 presentation (.ppt,pdf)
- UT Austin and UCSF 2004 project (coliroids)
- Find descriptions of 2005 projects