IGEM:MIT/2006/Introduction

From OpenWetWare

< IGEM:MIT | 2006
Revision as of 18:52, 25 May 2006 by Barry Canton (Talk | contribs)
Jump to: navigation, search

This page contains links to introductory information for the MIT iGEM team.

Contents

Lab safety

All people working in a biological research lab must undergo safety training.

To complete your online safety training, visit the Endy lab safety training guide. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.)

Heather will be giving the lab-specific training.

Wiki

How-to on wiki's

  • Getting started --> Quick tutorial on using the OpenWetWare wiki
  • Populate the official iGEM wiki

Abstraction hierarchy

  • PoPS and composability

Registry

  • Obtain accounts
  • Making a part (both basic and subpart)
  • BLAST
  • Sequence alignments

BioBricks assembly

  • BioBricks
  • Standard assembly
  • 3A assembly
  • In frame assembly
  • Direct synthesis

Part and device characterization

  • Plate reader characterization (i.e. Receiver)
  • Flow cytometry (i.e. Screening plasmid)
  • Microscopy
  • RT-PCR and Westerns

Searching and reading the literature

Lab techniques

Links

General introduction to ideas in Synthetic Biology

  1. Adventures in synthetic biology comic --> Learn about PoPS
  2. Foundations for engineering biology --> rant by Drew Endy
  3. MIT iGEM 2004 presentation (.ppt,pdf)
  4. UT Austin and UCSF 2004 project (coliroids)
  5. Find descriptions of 2005 projects

Week 1

Goals -

  • Do one assembly stage
  • Make a biobrick
  • Necessary software - VectorNTI, Registry, Wiki
  • Literature searching

Day 1

Dry Lab

  • Safety training
  • Lab tour
  • Registry accounts

Wet Lab

  • Pick a gene and design primers
    • Introduces parts
  • Start overnights
    • Introduces sterile technique, culturing,

Day 2

Dry Lab

  • BioBricks Construction

Wet Lab

  • Prep & Digest
Personal tools