IGEM:MIT/2006/Notebook/2006-10-10: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(→ALL) |
||
| (8 intermediate revisions by 2 users not shown) | |||
| Line 2: | Line 2: | ||
#Team meeting: 1pm | #Team meeting: 1pm | ||
#*assess weekend lab progress | #*assess weekend lab progress | ||
#**Got 2 LCs of something that might be 400 in top10; miniprepping today, going to sequence | |||
#**the "banana" cells (250 + (250+400)) from the sketchy colony grew up yellowish and weird colored...contamination | |||
#**The "mint" IK cells didn't smell so good...we're not sure why. maybe something is wrong with our IK cells? the transformed colonies weren't all big and spread out like IK normally are. we're miniprepping one of the LCs today. | |||
#*check in with advisors about biobricked yeast vector options | #*check in with advisors about biobricked yeast vector options | ||
#*make new deadlines (poster, practicing presentation, judge bribes, etc.) | #*make new deadlines (poster, practicing presentation, registry, judge bribes, etc.) | ||
#*assign 'out of lab' duties specifically | #*assign 'out of lab' duties specifically | ||
#*talk about distribution of smell @ igem issue, ask Reshma if she has any more info. | #*talk about distribution of smell @ igem issue, ask Reshma if she has any more info. | ||
#BMES meeting in afternoon | #*smell characterization | ||
#BMES meeting in afternoon 6-7 | |||
==also== | ==also== | ||
#smell | #smell LCs | ||
#*miniprep/sequence of good | |||
#*repeat ligation/transformation of bad | |||
==Parts that '''Need''' Numbers== | |||
osmY.E0840 | |||
osmY.Q04400.E0840 | |||
osmY.Q04400.J45199 | |||
osmY.Q04400.J45119 | |||
Q04400.J45199 | |||
Q04400.E0840 | |||
J45120+J45320 -> '''J45700''' | |||
J45250+J45400 -> '''J45800''' | |||
'''bolded''' numbers are the proposed numbers | |||
Latest revision as of 11:10, 10 October 2006
ALL
- Team meeting: 1pm
- assess weekend lab progress
- Got 2 LCs of something that might be 400 in top10; miniprepping today, going to sequence
- the "banana" cells (250 + (250+400)) from the sketchy colony grew up yellowish and weird colored...contamination
- The "mint" IK cells didn't smell so good...we're not sure why. maybe something is wrong with our IK cells? the transformed colonies weren't all big and spread out like IK normally are. we're miniprepping one of the LCs today.
- check in with advisors about biobricked yeast vector options
- make new deadlines (poster, practicing presentation, registry, judge bribes, etc.)
- assign 'out of lab' duties specifically
- talk about distribution of smell @ igem issue, ask Reshma if she has any more info.
- smell characterization
- assess weekend lab progress
- BMES meeting in afternoon 6-7
also
- smell LCs
- miniprep/sequence of good
- repeat ligation/transformation of bad
Parts that Need Numbers
osmY.E0840
osmY.Q04400.E0840
osmY.Q04400.J45199
osmY.Q04400.J45119
Q04400.J45199
Q04400.E0840
J45120+J45320 -> J45700
J45250+J45400 -> J45800
bolded numbers are the proposed numbers
