IGEM:MIT/2006/Notebook/2006-10-9: Difference between revisions
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==to do== | ==to do== | ||
LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) | #LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) | ||
*pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!] | #*pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!] | ||
#*('''AG''') I don't know if it is such a good idea to start them so early. Is anyone gonna be around later in the day? I'm coming in, hopefully, around 9AM. -André | |||
#*('''KB''')I made the LCs at 1 pm. transformants were scarce, but made 8 "mint" IK LCs and 1 sketchy "banana" top10 LC. | |||
#isolate the IAGD: '''Veena''' | |||
#*plate a few cells from each colony of transformants on an A/C plate | |||
#*LC the colonies that didn't grow on the A/C plate | |||
#**('''KB''') there are only 3 transformants. I am going to LC each in Kan and A/C (rather than plate). We will know tomorrow if any are Kan^+ and A/C^-, and this way you won't need to come back in today to do the 3 hour plating/growing step. | |||
==List of Parts/Numbers== | |||
* | |||
* | '''396''': 30.BAT2 (w/ B mut) | ||
'''397''': 30.BAT2.30 (w/ B mut) | |||
'''398''': 30.BAT2.30.THI3 (w/ B, T muts) | |||
*('''KB'''): we actually never mutated the T ''(successfully)'' | |||
'''399''': 30.BAT2.30.THI3.15 (w/ B, T muts) | |||
*('''KB'''): we never attached a terminator/made this part | |||
'''400''': Prom.30.BAT2.30.THI3.15 (w/ B, T muts) | |||
*('''KB'''): we actually never mutated the T ''(successfully)'' | |||
'''298''': pchBA.15 (w/ pA mut) | |||
'''299''': 30.pchBA.15 (w/ pA mut) | |||
'''300''': Prom.30.pchBA.15 (w/ pA mut) | |||
'''319''': 32.pchBA.15 | |||
'''320''': Prom.32.pchBA.15 | |||
Are the glycerols the only parts that we actually have? | |||
*('''KB'''): yes. make sure to check the new box for more recent parts (like inverter stuff, etc.) | |||
Also, whenever I streak these, should I just streak the IKs if there is an IK version and non-IK version? | |||
*('''KB'''): isn't megean just going to extract the dna?? so does it matter? i'd say use IK anyway though. | |||
==General Part Status Report== | |||
It makes a lot more sense for this to be done in Excel. I will do this and put it on in a bit. -André | |||
*('''KB'''): cool. thanks! |
Latest revision as of 10:55, 9 October 2006
to do
- LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : )
- pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!]
- (AG) I don't know if it is such a good idea to start them so early. Is anyone gonna be around later in the day? I'm coming in, hopefully, around 9AM. -André
- (KB)I made the LCs at 1 pm. transformants were scarce, but made 8 "mint" IK LCs and 1 sketchy "banana" top10 LC.
- isolate the IAGD: Veena
- plate a few cells from each colony of transformants on an A/C plate
- LC the colonies that didn't grow on the A/C plate
- (KB) there are only 3 transformants. I am going to LC each in Kan and A/C (rather than plate). We will know tomorrow if any are Kan^+ and A/C^-, and this way you won't need to come back in today to do the 3 hour plating/growing step.
List of Parts/Numbers
396: 30.BAT2 (w/ B mut)
397: 30.BAT2.30 (w/ B mut)
398: 30.BAT2.30.THI3 (w/ B, T muts)
- (KB): we actually never mutated the T (successfully)
399: 30.BAT2.30.THI3.15 (w/ B, T muts)
- (KB): we never attached a terminator/made this part
400: Prom.30.BAT2.30.THI3.15 (w/ B, T muts)
- (KB): we actually never mutated the T (successfully)
298: pchBA.15 (w/ pA mut)
299: 30.pchBA.15 (w/ pA mut)
300: Prom.30.pchBA.15 (w/ pA mut)
319: 32.pchBA.15
320: Prom.32.pchBA.15
Are the glycerols the only parts that we actually have?
- (KB): yes. make sure to check the new box for more recent parts (like inverter stuff, etc.)
Also, whenever I streak these, should I just streak the IKs if there is an IK version and non-IK version?
- (KB): isn't megean just going to extract the dna?? so does it matter? i'd say use IK anyway though.
General Part Status Report
It makes a lot more sense for this to be done in Excel. I will do this and put it on in a bit. -André
- (KB): cool. thanks!