IGEM:MIT/2006/Notebook/2006-10-9: Difference between revisions
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==to do== | ==to do== | ||
LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) '''anyone want to do this?''' | |||
#*pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!] | #*pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!] | ||
isolate the IAGD: '''Veena''' | |||
#*plate a few cells from each colony of transformants on an A/C plate | #*plate a few cells from each colony of transformants on an A/C plate | ||
#*LC the colonies that didn't grow on the A/C plate | #*LC the colonies that didn't grow on the A/C plate | ||
Revision as of 15:30, 8 October 2006
to do
LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) anyone want to do this?
- pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!]
isolate the IAGD: Veena
- plate a few cells from each colony of transformants on an A/C plate
- LC the colonies that didn't grow on the A/C plate
