IGEM:MIT/2006/Notebook/2006-10-9

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==to do==
==to do==
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#*LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) --anyone want to do this?
+
#LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) --anyone want to do this?
*pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK).  we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them.  [i REALLY hope there are transformants this time!!]
*pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK).  we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them.  [i REALLY hope there are transformants this time!!]
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#*isolate the IAGD: --veena
+
#isolate the IAGD: --veena
*plate a few cells from each colony of transformants on an A/C plate
*plate a few cells from each colony of transformants on an A/C plate
*LC the colonies that didn't grow on the A/C plate
*LC the colonies that didn't grow on the A/C plate

Revision as of 18:29, 8 October 2006

to do

  1. LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) --anyone want to do this?
  • pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!]
  1. isolate the IAGD: --veena
  • plate a few cells from each colony of transformants on an A/C plate
  • LC the colonies that didn't grow on the A/C plate
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