IGEM:MIT/2006/Notebook/2006-10-9

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(to do)
(to do)
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LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) '''anyone want to do this?'''
LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) '''anyone want to do this?'''
-
#*pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK).  we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them.  [i REALLY hope there are transformants this time!!]
+
*pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK).  we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them.  [i REALLY hope there are transformants this time!!]
isolate the IAGD: '''Veena'''
isolate the IAGD: '''Veena'''
-
#*plate a few cells from each colony of transformants on an A/C plate
+
*plate a few cells from each colony of transformants on an A/C plate
-
#*LC the colonies that didn't grow on the A/C plate
+
*LC the colonies that didn't grow on the A/C plate

Revision as of 18:30, 8 October 2006

to do

LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) anyone want to do this?

  • pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!]

isolate the IAGD: Veena

  • plate a few cells from each colony of transformants on an A/C plate
  • LC the colonies that didn't grow on the A/C plate
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