IGEM:MIT/2006/Notebook/2006-10-9: Difference between revisions
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'''396''': 30.BAT2 (w/ B mut) | '''396''': 30.BAT2 (w/ B mut) | ||
'''397''': 30.BAT2.30 (w/ B mut) | '''397''': 30.BAT2.30 (w/ B mut) | ||
'''398''': 30.BAT2.30.THI3 (w/ B, T muts) | '''398''': 30.BAT2.30.THI3 (w/ B, T muts) | ||
'''399''': 30.BAT2.30.THI3.15 (w/ B, T muts) | '''399''': 30.BAT2.30.THI3.15 (w/ B, T muts) | ||
'''400''': Prom.30.BAT2.30.THI3.15 (w/ B, T muts) | '''400''': Prom.30.BAT2.30.THI3.15 (w/ B, T muts) | ||
'''298''': pchBA.15 (w/ pA mut) | '''298''': pchBA.15 (w/ pA mut) | ||
'''299''': 30.pchBA.15 (w/ pA mut) | '''299''': 30.pchBA.15 (w/ pA mut) | ||
'''300''': Prom.30.pchBA.15 (w/ pA mut) | '''300''': Prom.30.pchBA.15 (w/ pA mut) | ||
'''319''': 32.pchBA.15 | '''319''': 32.pchBA.15 | ||
'''320''': Prom.32.pchBA.15 | '''320''': Prom.32.pchBA.15 | ||
Revision as of 08:51, 9 October 2006
to do
LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) On second thought, I don't know if it is such a good idea to start them so early. Is anyone gonna be around later in the day? I'm coming in, hopefully, around 9AM. -André
- pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!]
isolate the IAGD: Veena
- plate a few cells from each colony of transformants on an A/C plate
- LC the colonies that didn't grow on the A/C plate
List of Parts/Numbers
396: 30.BAT2 (w/ B mut)
397: 30.BAT2.30 (w/ B mut)
398: 30.BAT2.30.THI3 (w/ B, T muts)
399: 30.BAT2.30.THI3.15 (w/ B, T muts)
400: Prom.30.BAT2.30.THI3.15 (w/ B, T muts)
298: pchBA.15 (w/ pA mut)
299: 30.pchBA.15 (w/ pA mut)
300: Prom.30.pchBA.15 (w/ pA mut)
319: 32.pchBA.15
320: Prom.32.pchBA.15
Are the glycerols the only parts that we actually have?
Also, whenever I streak these, should I just streak the IKs if there is an IK version and non-IK version?