IGEM:MIT/2006/Notebook/2006-10-9

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to do

LC the "banana cells" and the "mint cells"...cells w/ both precursor & product generation!! : ) On second thought, I don't know if it is such a good idea to start them so early. Is anyone gonna be around later in the day? I'm coming in, hopefully, around 9AM. -André

  • pick colonies and LC from the 2 ligations above...should be 8 plates to LC (4 in top10 and 4 in IK). we probably want to do a ridiculous amount of LCs from the 250/400 plates b/c there probably won't be very many that are correct, and we'll instantly be able to tell which are right and which are wrong so it doesn't hurt to do a lot of them. [i REALLY hope there are transformants this time!!]

isolate the IAGD: Veena

  • plate a few cells from each colony of transformants on an A/C plate
  • LC the colonies that didn't grow on the A/C plate


List of Parts/Numbers

396: 30.BAT2 (w/ B mut) 397: 30.BAT2.30 (w/ B mut) 398: 30.BAT2.30.THI3 (w/ B, T muts) 399: 30.BAT2.30.THI3.15 (w/ B, T muts) 400: Prom.30.BAT2.30.THI3.15 (w/ B, T muts)

298: pchBA.15 (w/ pA mut) 299: 30.pchBA.15 (w/ pA mut) 300: Prom.30.pchBA.15 (w/ pA mut) 319: 32.pchBA.15 320: Prom.32.pchBA.15

Are the glycerols the only parts that we actually have?

Also, whenever I streak these, should I just streak the IKs if there is an IK version and non-IK version?

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