IGEM:MIT/2006/Notebook/2006-6-12: Difference between revisions
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#*Used 2xYT | #*Used 2xYT | ||
#*Plated 200μL cells | #*Plated 200μL cells | ||
#For TOP10 cells use this [https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&objectId=E62FEAF0F3C8B0E215833D3E8A49BD5D&treeNodeId=289731BD016F54BEBFEAA20F29AA1796 transformation protocol], which is similar but slightly different from the what we did [[Transforming chemically competent cells | previously]] | #For TOP10 cells use this [https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&objectId=E62FEAF0F3C8B0E215833D3E8A49BD5D&treeNodeId=289731BD016F54BEBFEAA20F29AA1796 transformation protocol], which is similar but slightly different from the what we did [[Transforming chemically competent cells | previously]] | ||
Revision as of 08:34, 12 June 2006
T-Shirt
WE NEED A T-SHIRT DESIGN!
06/12/06 Repeat (from 6/09/06) Transformation Lab
Sources
- Note, used 1/2 of designated source
- SAMT in PET28 1.6 μg
- BAMT in PET28 2.0 μg
- BSMT in PET28 2.0 μg
Dehydrated DNA Recovery (from paper)
- Final DNA Concentration: 1ng/μL (room for better quantification)
- Add appropriate amount of TE buffer to 1.7mL Eppendorf tubes (500μL for BAMT and BSMT; 400μL for SAMT).
- Carefully cut out paper that contains DNA and place into Eppen.
- Ensure submergence with toothpick
- Wait for 10 minutes
Transformation
- Followed chemical transformation protocol listed here.
- Used 2μL of plasmid/DNA.
- Heat shock the BL21(DL3) cells
- Used 2xYT
- Plated 200μL cells
- For TOP10 cells use this transformation protocol, which is similar but slightly different from the what we did previously
