IGEM:MIT/2006/Notebook/2006-6-12: Difference between revisions

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Revision as of 07:59, 12 June 2006

06/09/06 Preliminary Transformation Lab

Sources

  • Note, used 1/2 of designated source
  1. SAMT in PET28 1.6 μg
  2. BAMT in PET28 2.0 μg
  3. BSMT in PET28 2.0 μg

Dehydrated DNA Recovery (from paper)

  • Final DNA Concentration: 1ng/μL
  1. Add appropriate amount of TE buffer to 1.7mL Eppendorf tubes (500μL for BAMT and BSMT; 400μL for SAMT).
  2. Carefully cut out paper that contains DNA and place into Eppen.
    • Ensure submergence with toothpick
    • Wait for 10 minutes

Transformation

  1. Followed chemical transformation protocol listed here.
    • Used 2μL of plasmid/DNA.
    • Did not do heat shock
    • Used 2xYT
    • Plated 200μL cells
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