IGEM:MIT/2006/Notebook/2006-6-13: Difference between revisions
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==06/13/06 Lab== | |||
#Made glycerols of BAMT and SAMT from BL21 (6/9/06) | |||
#*Made two copies (4 total) of each by adding 1ml of each to glycerol | |||
==To do== | ==To do== | ||
#Save glycerols of all cultures | #Save glycerols of all cultures | ||
Revision as of 08:23, 13 June 2006
06/13/06 Lab
- Made glycerols of BAMT and SAMT from BL21 (6/9/06)
- Made two copies (4 total) of each by adding 1ml of each to glycerol
To do
- Save glycerols of all cultures
- Miniprep two colonies of each construct
- Submit for sequencing with T7 forward and reverse primers (need to find primers).
- Check for transformants.
- If there are transformants, start cultures of colonies from each strain and construct combination.
Notes
Sequencing
- pET28a(+) genbank file from Novagen <- Is this is the vector that the constructs came in?
- This vector has a T7 promoter primer binding site but no T3, SP6, M13 Forward or M13 Reverse primer binding site.
- Does anyone have a T7 terminator primer?
