IGEM:MIT/2006/Notebook/2006-6-16: Difference between revisions
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#Looking over all sequences, BAMT and BSMT were all fine- will use A colonies for PCR | #Looking over all sequences, BAMT and BSMT were all fine- will use A colonies for PCR | ||
#SAMT with reverse primer had sequencing issues on all colonies, with poor match and a shorter number of bases | #SAMT with reverse primer had sequencing issues on all colonies, with poor match and a shorter number of bases | ||
==Indole== | |||
==On order== | ==On order== | ||
Revision as of 08:47, 16 June 2006
Progress from Previous Day
- Looking over all sequences, BAMT and BSMT were all fine- will use A colonies for PCR
- SAMT with reverse primer had sequencing issues on all colonies, with poor match and a shorter number of bases
Indole
On order
Salicylic acid had been ordered, should be here any time now. Methyl salicylate and methyl benzoate have been ordered and should be here on Monday. Indole has been ordered, not sure when it is expected.
Todo
- PCR biobricks [SAMT, BAMT, BSMT...the sequences from the plasmids that were transformed into Top10 cells came out OK]
- Mutagenesis PCR for ATF1
- Transform plasmids isolated from Top10 cells into BL21 competent cells (and make our own BL21 competent cells)
- If we get methyl benzoate or methyl salicylate, try to determine the concentration necessary to detect a scent.
Wiki Development
Currently building a new page that is topical instead of linear, [[../../Index]]
