IGEM:MIT/2006/Notebook/2006-6-16: Difference between revisions
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Salicylic acid had been ordered, should be here any time now. Methyl salicylate and methyl benzoate have been ordered and should be here on Monday. Indole has been ordered, not sure when it is expected. | Salicylic acid had been ordered, should be here any time now. Methyl salicylate and methyl benzoate have been ordered and should be here on Monday. Indole has been ordered, not sure when it is expected. | ||
==Todo== | |||
#PCR biobricks [SAMT, BAMT, BSMT...the sequences from the plasmids that were transformed into Top10 cells came out OK] | #PCR biobricks [SAMT, BAMT, BSMT...the sequences from the plasmids that were transformed into Top10 cells came out OK] | ||
#Mutagenesis PCR for ATF1 | #Mutagenesis PCR for ATF1 | ||
#Transform plasmids isolated from Top10 cells into BL21 competent cells (and make our own BL21 competent cells) | #Transform plasmids isolated from Top10 cells into BL21 competent cells (and make our own BL21 competent cells) | ||
#If we get methyl benzoate or methyl salicylate, try to determine the concentration necessary to detect a scent. | #If we get methyl benzoate or methyl salicylate, try to determine the concentration necessary to detect a scent. | ||
Revision as of 07:13, 16 June 2006
On order
Salicylic acid had been ordered, should be here any time now. Methyl salicylate and methyl benzoate have been ordered and should be here on Monday. Indole has been ordered, not sure when it is expected.
Todo
- PCR biobricks [SAMT, BAMT, BSMT...the sequences from the plasmids that were transformed into Top10 cells came out OK]
- Mutagenesis PCR for ATF1
- Transform plasmids isolated from Top10 cells into BL21 competent cells (and make our own BL21 competent cells)
- If we get methyl benzoate or methyl salicylate, try to determine the concentration necessary to detect a scent.
