IGEM:MIT/2006/Notebook/2006-6-16

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Progress from Previous Day

  1. Looking over all sequences, BAMT and BSMT were all fine- will use A colonies for PCR
  2. SAMT with reverse primer had sequencing issues on all colonies, with poor match and a shorter number of bases

Indole

Here is a paper complete with GenBank Nos. for the e. coli gene we would need to mutate in order to knock-out its indole-producing trait: http://www.pubmedcentral.gov/articlerender.fcgi?artid=523188

On order

Salicylic acid had been ordered, should be here any time now. Methyl salicylate and methyl benzoate have been ordered and should be here on Monday. Indole has been ordered, not sure when it is expected.

Toxicity

Todo

  1. PCR biobricks [SAMT, BAMT, BSMT...the sequences from the plasmids that were transformed into Top10 cells came out OK]
  2. Mutagenesis PCR for ATF1
  3. Transform plasmids isolated from Top10 cells into BL21 competent cells (and make our own BL21 competent cells)
  4. If we get methyl benzoate or methyl salicylate, try to determine the concentration necessary to detect a scent.

Wiki Development

Currently building a new page that is topical instead of linear, [[../../Index]]

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