IGEM:MIT/2006/Notebook/2006-6-17: Difference between revisions
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#check BL21 plates for transformants | #check BL21 plates for transformants -- '''TONS of transformants! They were borderline overgrown, even in the 20μL plates. I put them in the fridge; I will start liquid cultures sometime this afternoon.''' | ||
#make | #make D, E, F liquid cultures in LB Kan from each plate (assuming possible) | ||
#put in 37c room with shaking for about 16 hours | #put in 37c room with shaking for about 16 hours | ||
#will remove next am | #will remove next am | ||
Revision as of 07:37, 17 June 2006
am
- check BL21 plates for transformants -- TONS of transformants! They were borderline overgrown, even in the 20μL plates. I put them in the fridge; I will start liquid cultures sometime this afternoon.
- make D, E, F liquid cultures in LB Kan from each plate (assuming possible)
- put in 37c room with shaking for about 16 hours
- will remove next am
