IGEM:MIT/2006/Notebook/2006-6-17

am

1. check BL21 plates for transformants -- TONS of transformants! They were borderline overgrown, even in the 20μL plates. I put them in the fridge; I will start liquid cultures sometime this afternoon.
2. make D, E, F liquid cultures in LB Kan from each plate (assuming possible)
3. put in 37c room with shaking for about 16 hours
4. will remove next am