IGEM:MIT/2006/Notebook/2006-6-20: Difference between revisions

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*Plated liquid cultures onto M9 with 50, 100, 200 μL SA
*Plated liquid cultures onto M9 with 50, 100, 200 μL SA
*2 sets of 4 (BSMT M9, BSMT LB, SAMT M9, SAMT LB) with one set of 3 missing BSMT M9
**2 sets of 4 (BSMT M9, BSMT LB, SAMT M9, SAMT LB) with one set of 3 missing BSMT M9


==Repeat Smell Experiment==
==Repeat Smell Experiment==

Revision as of 08:22, 20 June 2006

To do

  1. Set up and run gradient PCR for BAMT and SAMT
  2. run test gels on exhaustive BAMT and SAMT pcr products - possibly reconsider primers
  3. Reconsider ATF1 primers definitely
  4. Grow colonies on sketchy minimal media plates
  5. Smell LB liquid and minimal liquid cultures
  6. Pour new minimal plates
  7. Get in touch with GC lab
          • 3 pm: Stephen has meeting --- light sensor part!

WINTERGREEN!!!!!!!

  • The liquid cultures from yesterday SMELLED LIKE WINTERGREEN!! SO EXCITING!!!! YAY!
  • The SAMT+SA and the BSMT+SA worked, in both LB and minimal media
  • Now it's time to optimize SA concentration :)
  • Plated liquid cultures onto M9 with 50, 100, 200 μL SA
    • 2 sets of 4 (BSMT M9, BSMT LB, SAMT M9, SAMT LB) with one set of 3 missing BSMT M9

Repeat Smell Experiment

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