IGEM:MIT/2006/Notebook/2006-6-20: Difference between revisions
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#run test gels on exhaustive BAMT and SAMT pcr products - possibly reconsider primers | #run test gels on exhaustive BAMT and SAMT pcr products - possibly reconsider primers | ||
#Reconsider ATF1 primers definitely | #Reconsider ATF1 primers definitely | ||
#Get in touch with GC lab | #Get in touch with GC lab | ||
#****3 pm: Stephen has meeting --- light sensor part! | #****3 pm: Stephen has meeting --- light sensor part! | ||
Revision as of 08:28, 20 June 2006
To do
- Set up and run gradient PCR for BAMT and SAMT
- run test gels on exhaustive BAMT and SAMT pcr products - possibly reconsider primers
- Reconsider ATF1 primers definitely
- Get in touch with GC lab
- 3 pm: Stephen has meeting --- light sensor part!
WINTERGREEN!!!!!!!
- The liquid cultures from yesterday SMELLED LIKE WINTERGREEN!! SO EXCITING!!!! YAY!
- The SAMT+SA and the BSMT+SA worked, in both LB and minimal media
- Now it's time to optimize SA concentration :)
- Plated liquid cultures onto M9 with 50, 100, 200 μL SA
- 2 sets of 4 (BSMT M9, BSMT LB, SAMT M9, SAMT LB) with one set of 3 missing BSMT M9
Repeat Smell Experiment
| Control | BAMT/BA | SAMT/SA | BSMT/BA | BSMT/SA |
|---|---|---|---|---|
| 10mL LB + 20μL BL21 cells + 8.2μL BA [in a tube] | 10mL LB Kan + 20μL BAMT cells + 8.2μL BA [in a tube] | 10mL LB Kan + 20μL SAMT cells + 6.2μL SA [in a tube] | 10mL LB Kan + 20μL BSMT cells + 8.2μL BA [in a tube] | 10mL LB Kan + 20μL BSMT cells + 6.2μL SA [in a tube] |
| 10mL minimal media + 20μL BL21 cells + 8.2μL BA [in a tube] | 10mL minimal media Kan + 20μL BAMT cells + 8.2μL BA [in a tube] | 10mL minimal media Kan + 20μL SAMT cells + 6.2μL SA [in a tube] | 10mL minimal media Kan + 20μL BSMT cells + 8.2μL BA [in a tube] | 10mL minimal media Kan + 20μL BSMT cells + 6.2μL SA [in a tube] |
