IGEM:MIT/2006/Notebook/2006-6-20

To do

1. Set up and run gradient PCR for BAMT and SAMT
2. run test gels on exhaustive BAMT and SAMT pcr products - possibly reconsider primers
3. Reconsider ATF1 primers definitely
4. Pour new minimal plates
5. Get in touch with GC lab
   • • • • 3 pm: Stephen has meeting --- light sensor part!

WINTERGREEN!!!!!!!

1. The liquid cultures from yesterday SMELLED LIKE WINTERGREEN!! SO EXCITING!!!! YAY!
2. The SAMT+SA and the BSMT+SA worked, in both LB and minimal media
   • Now it's time to optimize SA concentration :)
3. Plated liquid cultures onto M9 with 50, 100, 200 μL SA
   • 2 sets of 4 (BSMT M9, BSMT LB, SAMT M9, SAMT LB) with one set of 3 missing BSMT M9

Repeat Smell Experiment

Control	BAMT/BA	SAMT/SA	BSMT/BA	BSMT/SA
10mL LB + 20μL BL21 cells + 8.2μL BA [in a tube]	10mL LB Kan + 20μL BAMT cells + 8.2μL BA [in a tube]	10mL LB Kan + 20μL SAMT cells + 6.2μL SA [in a tube]	10mL LB Kan + 20μL BSMT cells + 8.2μL BA [in a tube]	10mL LB Kan + 20μL BSMT cells + 6.2μL SA [in a tube]
10mL minimal media + 20μL BL21 cells + 8.2μL BA [in a tube]	10mL minimal media Kan + 20μL BAMT cells + 8.2μL BA [in a tube]	10mL minimal media Kan + 20μL SAMT cells + 6.2μL SA [in a tube]	10mL minimal media Kan + 20μL BSMT cells + 8.2μL BA [in a tube]	10mL minimal media Kan + 20μL BSMT cells + 6.2μL SA [in a tube]