IGEM:MIT/2006/Notebook/2006-6-20

To do

1. Possibly reconsider BAMT/SAMT primers
2. Reconsider ATF1 primers definitely
3. Get in touch with GC lab
   • • • • 3 pm: Stephen has meeting --- light sensor part!

WINTERGREEN!!!!!!!

1. The liquid cultures from yesterday SMELLED LIKE WINTERGREEN!! SO EXCITING!!!! YAY!
2. The SAMT+SA and the BSMT+SA worked, in both LB and minimal media
   • Now it's time to optimize SA concentration :)
3. Plated liquid cultures onto M9 with 50, 100, 200 μL SA
   • 2 sets of 4 (BSMT M9, BSMT LB, SAMT M9, SAMT LB) with one set of 3 missing BSMT M9

Indole

1. Indole from [[../2006-6-19 |6-19-2006]] definitely was root of bad bacterial smell.

Repeat Smell Experiment

Control	BAMT/BA	SAMT/SA	BSMT/BA	BSMT/SA
10mL LB + 20μL BL21 cells + 8.2μL BA [in a tube]	10mL LB Kan + 20μL BAMT cells + 8.2μL BA [in a tube]	10mL LB Kan + 20μL SAMT cells + 6.2μL SA [in a tube]	10mL LB Kan + 20μL BSMT cells + 8.2μL BA [in a tube]	10mL LB Kan + 20μL BSMT cells + 6.2μL SA [in a tube]
10mL minimal media + 20μL BL21 cells + 8.2μL BA [in a tube]	10mL minimal media Kan + 20μL BAMT cells + 8.2μL BA [in a tube]	10mL minimal media Kan + 20μL SAMT cells + 6.2μL SA [in a tube]	10mL minimal media Kan + 20μL BSMT cells + 8.2μL BA [in a tube]	10mL minimal media Kan + 20μL BSMT cells + 6.2μL SA [in a tube]

Repeat BAMT/SAMT PCR

1. 16 sample tubes, 6 controls (8, 3 of each)
   • (-): no template- just primers, (-): no primer- just plasmid, (+): new primer/template
2. Sample master mix 18x, control master mix 10x, [[../2006-6-15 | from here]]
3. Temperature gradient from 42-54