IGEM:MIT/2006/Notebook/2006-6-29

PCR cleanup / Nanodrop / ran gel - TAKE 3

1. PCR cleanup of BSMT and Barry's part: followed standard protocol for PCR cleanup...not too exciting : ) Just remember to elute with 30μL of the last buffer.
2. Nanodrop: check the concentrations of the cleaned-up cut DNA, and of the cut backbone:
   • Plasmid (Amp resistance, cut with EcoRI and PstI): ? ng/μL
   • Barry's part (PSBI82E0040, cut with EcoRI and PstI): ? ng/μL
   • BSMT (cut with EcoRI, PstI, and DpnI): ? ng/μL
3. Gel: the columns, from left to right, are:
   • 2log ladder
   • cut BSMT
   • cut Barry's biobrick part
   • cut backbone
   • uncut backbone