IGEM:MIT/2006/Notebook/2006-6-30: Difference between revisions
No edit summary |
|||
| Line 2: | Line 2: | ||
Two plates carrying the control to compare with the stationary phase promoter are in the 30 degree room. Remove them at the beginning of the day (really early if you don't want to come in on Saturday) and inoculate them in '''LB AMP''' liquid culture. Please remember to take them out. There is also a liquid culture labelled "control promoter" in the 37 degree room. I don't think it was growing yesterday, but you may want to check if it grew. If it grew, you still may want to make the LB AMP liquid culture from the fresh plates. If it didn't, I guess we have to make the liquid cultures from the 30 degree plates. If you guys feel ambitious and the liquid culture grew, you could start the fluorescent experiments (you can ask Barry to show you how it's done). Anyways, good luck, and have a great weekend! | Two plates carrying the control to compare with the stationary phase promoter are in the 30 degree room. Remove them at the beginning of the day (really early if you don't want to come in on Saturday) and inoculate them in '''LB AMP''' liquid culture. Please remember to take them out. There is also a liquid culture labelled "control promoter" in the 37 degree room. I don't think it was growing yesterday, but you may want to check if it grew. If it grew, you still may want to make the LB AMP liquid culture from the fresh plates. If it didn't, I guess we have to make the liquid cultures from the 30 degree plates. If you guys feel ambitious and the liquid culture grew, you could start the fluorescent experiments (you can ask Barry to show you how it's done). Anyways, good luck, and have a great weekend! | ||
==Results of 6/30 BSMT-BB Ligation/Transformation== | |||
{| border="1" cellpadding="5" | |||
!|Volume Cells Plated | |||
!|pUC19-Old | |||
!|pUC19-Fresh | |||
!|E0040BB (+Ctrl) | |||
!|BSMTBB | |||
|- | |||
|20 μL | |||
|10mL LB Kan + 20μL BAMT cells + 8.2μL BA [in a tube] | |||
|10mL LB Kan + 20μL SAMT cells + 6.2μL SA [in a tube] | |||
|10mL LB Kan + 20μL BSMT cells + 8.2μL BA [in a tube] | |||
|10mL LB Kan + 20μL BSMT cells + 6.2μL SA [in a tube] | |||
|- | |||
|10mL minimal media + 20μL BL21 cells + 8.2μL BA [in a tube] | |||
|10mL minimal media Kan + 20μL BAMT cells + 8.2μL BA [in a tube] | |||
|10mL minimal media Kan + 20μL SAMT cells + 6.2μL SA [in a tube] | |||
|10mL minimal media Kan + 20μL BSMT cells + 8.2μL BA [in a tube] | |||
|10mL minimal media Kan + 20μL BSMT cells + 6.2μL SA [in a tube] | |||
|- | |||
|} | |||
Revision as of 06:53, 30 June 2006
Stationary Phase Promoter Stuff To Get Done
Two plates carrying the control to compare with the stationary phase promoter are in the 30 degree room. Remove them at the beginning of the day (really early if you don't want to come in on Saturday) and inoculate them in LB AMP liquid culture. Please remember to take them out. There is also a liquid culture labelled "control promoter" in the 37 degree room. I don't think it was growing yesterday, but you may want to check if it grew. If it grew, you still may want to make the LB AMP liquid culture from the fresh plates. If it didn't, I guess we have to make the liquid cultures from the 30 degree plates. If you guys feel ambitious and the liquid culture grew, you could start the fluorescent experiments (you can ask Barry to show you how it's done). Anyways, good luck, and have a great weekend!
Results of 6/30 BSMT-BB Ligation/Transformation
| Volume Cells Plated | pUC19-Old | pUC19-Fresh | E0040BB (+Ctrl) | BSMTBB |
|---|---|---|---|---|
| 20 μL | 10mL LB Kan + 20μL BAMT cells + 8.2μL BA [in a tube] | 10mL LB Kan + 20μL SAMT cells + 6.2μL SA [in a tube] | 10mL LB Kan + 20μL BSMT cells + 8.2μL BA [in a tube] | 10mL LB Kan + 20μL BSMT cells + 6.2μL SA [in a tube] |
| 10mL minimal media + 20μL BL21 cells + 8.2μL BA [in a tube] | 10mL minimal media Kan + 20μL BAMT cells + 8.2μL BA [in a tube] | 10mL minimal media Kan + 20μL SAMT cells + 6.2μL SA [in a tube] | 10mL minimal media Kan + 20μL BSMT cells + 8.2μL BA [in a tube] | 10mL minimal media Kan + 20μL BSMT cells + 6.2μL SA [in a tube] |
