IGEM:MIT/2006/Notebook/2006-6-30
Stationary Phase Promoter Stuff To Get Done
Two plates carrying the control to compare with the stationary phase promoter are in the 30 degree room. Remove them at the beginning of the day (really early if you don't want to come in on Saturday) and inoculate them in LB AMP liquid culture. Please remember to take them out. There is also a liquid culture labelled "control promoter" in the 37 degree room. I don't think it was growing yesterday, but you may want to check if it grew. If it grew, you still may want to make the LB AMP liquid culture from the fresh plates. If it didn't, I guess we have to make the liquid cultures from the 30 degree plates. If you guys feel ambitious and the liquid culture grew, you could start the fluorescent experiments (you can ask Barry to show you how it's done). Anyways, good luck, and have a great weekend!
Number of Transformants from 6/29 BSMT-BB Ligation/Transformation
| Volume Cells Plated | pUC19-Old Top 10 | pUC19-Fresh Top 10 | E0040BB (+Ctrl) | BSMTBB |
|---|---|---|---|---|
| 20μL | 0 | 0 | 6 | 1 |
| 200μL | 2 | 20 | ~100 | 5 |
We can make a few conclusions from these data:
- The old Top 10 cells are probably not suitable for use (despite colonies appearing at the 200μ plating volume).
- Barry's E0040 positive control worked.
- Our ligation was "succcessful", as colonies did appear on our plates.
