IGEM:MIT/2006/Notebook/2006-6-6: Difference between revisions

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#*Post-translational modification
#*Post-translational modification
#*Material availability
#*Material availability
#Use cDNA from arabadopsis
#**Use cDNA from arabadopsis
#Reaction catalyzed
#Reaction catalyzed
#*Substrate
#*Substrate

Revision as of 11:54, 6 June 2006

A very brief description of the mechanism for making cinnamon smells:

http://docs.lib.purdue.edu/dissertations/AAI9939353/

We'll dump all this information on today's page for now but you guys should think about what page structure you want to use to store all your information!

Enzyme 1

  1. Species
  2. Sequence & Structure
    • Codon optimized?
    • Restriction sites
    • Post-translational modification
    • Material availability
      • cDNA from arabadopsis
      • Request from authors
        • Form of DNA?
      • need to synthesize?
  3. Reaction catalyzed
    • Substrate
      • Synthesized in our chassis or supplied exogenously?
    • Product
      • Coolness (Austin)
    • Reaction rates - Km,kcat
  4. Does it smell??
    • Regulation issues
    • Transport issues for substrate/product
  5. Ported to E. coli or yeast?
  6. What is the regulation in planta
  7. References & Links
    • Corresponding authors
    • Genbank accession numbers
    • Pubmed
    • Websites
    • Any companies or patents?


Kate's enzyme

  1. Species
  2. Sequence & Structure
    • Codon optimized?
    • Restriction sites
    • Post-translational modification
    • Material availability
      • cDNA from arabadopsis
      • Request from authors
        • Form of DNA?
      • need to synthesize?
  3. Reaction catalyzed
    • Substrate
      • Synthesized in our chassis or supplied exogenously?
    • Product
      • Coolness (Austin)
    • Reaction rates - Km,kcat
  4. Does it smell??
    • Regulation issues
    • Transport issues for substrate/product
  5. Ported to E. coli or yeast?
  6. What is the regulation in planta
  7. References & Links
    • Corresponding authors
    • Genbank accession numbers
    • Pubmed
    • Websites
    • Any companies or patents?

Jasmonic acid carboxyl methyltransferase

  1. Species: Arabidopsis thaliana (thale cress)
  2. Sequence & Structure
    • Documented to work in E. coli
    • Restriction sites
    • Post-translational modification
    • Material availability
      • Use cDNA from arabadopsis
  3. Reaction catalyzed
    • Substrate
      • Synthesized in our chassis or supplied exogenously?
    • Product
      • Coolness (Austin)
    • Reaction rates - Km,kcat
  4. Does it smell??
    • Regulation issues
    • Transport issues for substrate/product
  5. Ported to E. coli or yeast?
  6. What is the regulation in planta
  7. References & Links
    • Corresponding authors
    • Genbank accession numbers
    • Pubmed
    • Websites
    • Any companies or patents?

Enzyme 4

  1. Species
  2. Sequence & Structure
    • Codon optimized?
    • Restriction sites
    • Post-translational modification
    • Material availability
      • cDNA from arabadopsis
      • Request from authors
        • Form of DNA?
      • need to synthesize?
  3. Reaction catalyzed
    • Substrate
      • Synthesized in our chassis or supplied exogenously?
    • Product
      • Coolness (Austin)
    • Reaction rates - Km,kcat
  4. Does it smell??
    • Regulation issues
    • Transport issues for substrate/product
  5. Ported to E. coli or yeast?
  6. What is the regulation in planta
  7. References & Links
    • Corresponding authors
    • Genbank accession numbers
    • Pubmed
    • Websites
    • Any companies or patents?
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