IGEM:MIT/2006/Notebook/2006-6-6: Difference between revisions
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#*Product: Methyl jasmonate | #*Product: Methyl jasmonate | ||
#**Given off by plants as defense, also in jasmin oil | #**Given off by plants as defense, also in jasmin oil | ||
#*Reaction rates - K<sub>m</sub>: 38.5 µM; K<sub>cat</sub>:25 | #*Reaction rates - K<sub>m</sub>: 38.5 µM; K<sub>cat</sub>:25 S<super>−1</super> | ||
#Does it smell?? | #Does it smell?? | ||
#*Methyl jasmonate has jasmin smell | #*Methyl jasmonate has jasmin smell |
Revision as of 12:51, 6 June 2006
A very brief description of the mechanism for making cinnamon smells:
http://docs.lib.purdue.edu/dissertations/AAI9939353/
We'll dump all this information on today's page for now but you guys should think about what page structure you want to use to store all your information!
Enzyme 1
- Species
- Sequence & Structure
- Codon optimized?
- Restriction sites
- Post-translational modification
- Material availability
- cDNA from arabadopsis
- Request from authors
- Form of DNA?
- need to synthesize?
- Reaction catalyzed
- Substrate
- Synthesized in our chassis or supplied exogenously?
- Product
- Coolness (Austin)
- Reaction rates - Km,kcat
- Substrate
- Does it smell??
- Regulation issues
- Transport issues for substrate/product
- Ported to E. coli or yeast?
- What is the regulation in planta
- References & Links
- Corresponding authors
- Genbank accession numbers
- Pubmed
- Websites
- Any companies or patents?
Kate's enzyme
- Species
- Sequence & Structure
- Codon optimized?
- Restriction sites
- Post-translational modification
- Material availability
- cDNA from arabadopsis
- Request from authors
- Form of DNA?
- need to synthesize?
- Reaction catalyzed
- Substrate
- Synthesized in our chassis or supplied exogenously?
- Product
- Coolness (Austin)
- Reaction rates - Km,kcat
- Substrate
- Does it smell??
- Regulation issues
- Transport issues for substrate/product
- Ported to E. coli or yeast?
- What is the regulation in planta
- References & Links
- Corresponding authors
- Genbank accession numbers
- Pubmed
- Websites
- Any companies or patents?
Jasmonic acid carboxyl methyltransferase
- Species: Arabidopsis thaliana (thale cress)
- Sequence & Structure
- Use cDNA from A. thaliana (multistep- screened with Brassica NTR1)
- JMT cDNA inserted into the pGEX-2T vector (Amersham Pharmacia-GE) at the EcoRI site and fused with glutathione-S-transferase gene (GST).
- The recombinant was transformed into E. coli BL21. Fusion protein GST-JMT was expressed
- Purified to get JMT- issue?
- Reaction catalyzed
- Substrate: Jasmonic acid and SAM added exogenously
- Product: Methyl jasmonate
- Given off by plants as defense, also in jasmin oil
- Reaction rates - Km: 38.5 µM; Kcat:25 S<super>−1</super>
- Does it smell??
- Methyl jasmonate has jasmin smell
- Regulation issues
- Transport issues for substrate/product
- Ported to E. coli
- Regulated in planta:
- References & Links
- Corresponding authors
- Genbank accession numbers: BD441046, BD441047
PAL & SAM:CCMT
- Species: PAL (Phenylalanine Ammonia-lyase) is found in several organisms, but for our interests, it seems the best option is the gene from Rhodosporidium toruloides. SAM:CCMT (S-adenosyl-L-methionine:cinnamate carboxyl methyltransferase) can only be found in Ocimum basilicum L., Lamieaceae (a basil plant) as far as I can tell.
- Sequence & Structure
- Codon optimized?
- Restriction sites
- Post-translational modification
- Material availability
- cDNA from arabadopsis
- Request from authors
- Form of DNA?
- need to synthesize?
- Reaction catalyzed
- Substrate
- Synthesized in our chassis or supplied exogenously?
- Product
- Coolness (Austin)
- Reaction rates - Km,kcat
- Substrate
- Does it smell??
- Regulation issues
- Transport issues for substrate/product
- Ported to E. coli or yeast?
- What is the regulation in planta
- References & Links
- Corresponding authors
- Genbank accession numbers
- Pubmed
- Websites
- Any companies or patents?