IGEM:MIT/2006/Notebook/2006-6-7: Difference between revisions
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==Precursor Database== | ==Precursor Database== | ||
*Transport | *Transport | ||
**SA can be transported into/out of tobacco cells (<link http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html>) | **SA can be transported into/out of tobacco cells (<link>http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html</link>) | ||
*Biosynthesis (salicylic acid/benzoic acid): | *Biosynthesis (salicylic acid/benzoic acid): | ||
**http://www.hort.purdue.edu/rhodcv/hort640c/secprod/sec7.gif" | **http://www.hort.purdue.edu/rhodcv/hort640c/secprod/sec7.gif" | ||
Revision as of 14:11, 7 June 2006
Precursor Database
- Transport
- SA can be transported into/out of tobacco cells (<link>http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html</link>)
- Biosynthesis (salicylic acid/benzoic acid):
- Toxicity
- What ranges of concentrations should we be adding
Banana- 10-15 mM isoamyl alcohol
Product Database
Sequence analysis
- Restriction sites
- Codon optimization
- Registry accounts
- Install DNA 2.0 software?
Systems & Applications
Things to get
- Precursor molecules etc.
- BL21(DE3)+pLysS competent cells (the Endy Lab has these)
- Arab. JMT
Parts in the registry
<bbpart>BBa_J45000</bbpart> <bbpart>BBa_J45001</bbpart> <bbpart>BBa_J45002</bbpart> <bbpart>BBa_J45003</bbpart> <bbpart>BBa_J45004</bbpart> <bbpart>BBa_J45005</bbpart> <bbpart>BBa_J45006</bbpart> <bbpart>BBa_J45007</bbpart>
