IGEM:MIT/2006/Notebook/2006-6-7: Difference between revisions

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==Precursor Database==
==Compound Database==
*Transport
===Transport===
**SA can be transported into/out of tobacco cells (http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html)
*SA can be transported into/out of tobacco cells (http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html)
*Biosynthesis (salicylic acid/benzoic acid):
===Biosynthesis===
**http://www.hort.purdue.edu/rhodcv/hort640c/secprod/sec7.gif"
====salicylic acid/benzoic acid====
*Biosynthesis (jasmonic acid)
http://www.hort.purdue.edu/rhodcv/hort640c/secprod/sec7.gif
**Many pathways described at http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1066929&blobtype=pdf
====jasmonic acid====
*Toxicity
*Many pathways described at http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1066929&blobtype=pdf
*What ranges of concentrations should we be adding
===Toxicity===
Banana- 10 mM isoamyl alcohol
===Concentration===
What ranges of concentrations should we be adding?


Jasmine- 1 mM JA and 1 mM S-adenosyl methionine (SAM)
====banana====
 
*10 mM isoamyl alcohol
SAMT (A. Majus and C. breweri) 5 μg/ml of SA (or BA)
====jasmine====
 
*1 mM JA and 1 mM S-adenosyl methionine (SAM)
==Product Database==
====salicylic acid or benzoic acid====
*SAMT (A. Majus and C. breweri) 5 μg/ml of SA (or BA)


==Sequence analysis==
==Sequence analysis==

Revision as of 16:31, 7 June 2006

Compound Database

Transport

Biosynthesis

salicylic acid/benzoic acid

http://www.hort.purdue.edu/rhodcv/hort640c/secprod/sec7.gif

jasmonic acid

Toxicity

Concentration

What ranges of concentrations should we be adding?

banana

  • 10 mM isoamyl alcohol

jasmine

  • 1 mM JA and 1 mM S-adenosyl methionine (SAM)

salicylic acid or benzoic acid

  • SAMT (A. Majus and C. breweri) 5 μg/ml of SA (or BA)

Sequence analysis

  • Restriction sites
  • Codon optimization
  • Registry accounts
  • Install DNA 2.0 software?

Systems & Applications

Things to get

  • Precursor molecules etc.
  • BL21(DE3)+pLysS competent cells (the Endy Lab has these)
  • Arab. JMT

Parts in the registry

<bbpart>BBa_J45000</bbpart> <bbpart>BBa_J45001</bbpart> <bbpart>BBa_J45002</bbpart> <bbpart>BBa_J45003</bbpart> <bbpart>BBa_J45004</bbpart> <bbpart>BBa_J45005</bbpart> <bbpart>BBa_J45006</bbpart> <bbpart>BBa_J45007</bbpart> <bbpart>Bba_J45010</bbpart>

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