IGEM:MIT/2006/Notebook/2006-6-7: Difference between revisions

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===Transport===
===Transport===
*SA can be transported into/out of tobacco cells (http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html)
*SA can be transported into/out of tobacco cells (http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html)
*BA was added exogenously, and methyl benzoate (MB) was produced, so BA can enter ''E. coli''.  MB concentrations were measured without needing to lyse the cells, so MB '''can''' exit the cell.  ([[IGEM:MIT/2006/Notebook/2006-6-6==BAMT: SAM-Benzoic Acid Carboxyl Methyltransferase==|Reference]]; it is the first paper under this section)
*BA was added exogenously, and methyl benzoate (MB) was produced, so BA can enter ''E. coli''.  MB concentrations were measured without needing to lyse the cells, so MB '''can''' exit the cell.  ([[IGEM:MIT/2006/Notebook/2006-6-6#BAMT: SAM-Benzoic Acid Carboxyl Methyltransferase|Reference]]; it is the first paper under this section)


===Biosynthesis===  
===Biosynthesis===  

Revision as of 05:32, 8 June 2006

Compound Database

Transport

Biosynthesis

salicylic acid/benzoic acid

http://www.hort.purdue.edu/rhodcv/hort640c/secprod/sec7.gif

jasmonic acid

Toxicity

Concentration

What ranges of concentrations should we be adding?

banana

  • 10 mM isoamyl alcohol

jasmine

  • 1 mM JA and 1 mM S-adenosyl methionine (SAM)

salicylic acid or benzoic acid

  • SAMT (A. Majus and C. breweri) 5 μg/ml of SA (or BA)

Sequence analysis

  • Restriction sites
  • Codon optimization
  • Registry accounts
  • Install DNA 2.0 software?

Systems & Applications

Things to get

  • Precursor molecules etc.
  • BL21(DE3)+pLysS competent cells (the Endy Lab has these)
  • Arab. JMT

Parts in the registry

<bbpart>BBa_J45000</bbpart> <bbpart>BBa_J45001</bbpart> <bbpart>BBa_J45002</bbpart> <bbpart>BBa_J45003</bbpart> <bbpart>BBa_J45004</bbpart> <bbpart>BBa_J45005</bbpart> <bbpart>BBa_J45006</bbpart> <bbpart>BBa_J45007</bbpart> <bbpart>Bba_J45010</bbpart>

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